Presep® Fractionating Column Series
Presep® for medium-pressure fractionation and flash chromatography can be used in the medium-pressure fractionation / purification system of each company.
Silica gel series if you try fractionation first
- Presep® (Luer Lock) Silica Gel (HC-N) Column: Spherical silica gel packing agent that can hold and load the sample at a high level
- Presep® (Luer Lock) Silica Gel (SP) Column: Spherical silica gel packing agent that have standard hold and high reproducibility / resolution
- Presep® (Luer Lock) Silica Gel Column: Crushed silica gel packing agent (small particle size) showing excellent separation efficiency
Physical characteristics of filler
| Product Name | Shape | Particle Size (μm) | Pore Diameter (nm) | Pore Capacity (mL/g) | Specific Surface Area (m2/g) | pH |
|---|---|---|---|---|---|---|
| Presep® (Luer Lock) Silica Gel (HC-N) | Spherical | 35-63 | 4 | 0.5 | 780 | 6.5-7.5 |
| Presep® (Luer Lock) Silica Gel (SP) | Spherical | 40-64 | 6 | 0.75 | 475 | 6.5-7.5 |
| Presep® (Luer Lock) Silica Gel | Crushed | 20-40 | 7 | 0.8 | 450 | 5.5-7.5 |
Aminopropyl-modified silica gel series suitable for basic compounds
- Presep® (Luer Lock) NH2 (HC) Column: Spherical silica gel packing agents make it possible to perform separation and purification in normal-phase, reversed-phase, and ion exchange modes
- Presep® (Luer Lock) NH2 Column: Crushed silica gel showing the same separation mode as that of spherical silica gel
Physical characteristics of filler
| Product Name | Shape | Particle Size (μm) | Pore Diameter (nm) | Pore Capacity (mL/g) | Specific Surface Area (m2/g) |
|---|---|---|---|---|---|
| Presep® (Luer Lock) NH2 (HC) | Spherical | 36-63 | 4.5 | 1.0 | 900 |
| Presep® (Luer Lock) NH2 | Crushed | 38-63 | 6.5 | 0.7 | 460 |
Presep® (Luer Lock) Silica Gel (HC-N)
Presep® (Luer Lock) Silica Gel (HC-N) Column is a column packed with spherical silica gel with a large specific surface area and shows very high retention capability and resolution. As its effect, the quantity of sample that can be loaded by 1 purification operation is approximately twice that of the conventional product (compared with the conventional column of our company), and the cost and time required for fractionation can be reduced. Because of its high retention capability, an eluent with a high proportion of polar solvents can be used, and it is effective for purification of poorly soluble components. This product is also gentle to the environment, for example, by reducing the amount of solvent used and the number of columns discarded. TLC plates (Silica Gel 40F254 TLC Plate-Wako) are recommended for the examination of fractionation conditions.
Features
- High retention capability and resolution
- Increased sample load
- Effective for purification of poorly soluble components
Comparison of retention capability
Reference values of column size, recommended flow rate, and load
| Size (mm×mm) |
Filler Amount (g) |
Column Volume (mL) |
Recommended Flow Rate (mL/min.) |
Estimated Largest Load Value (g) | |||
|---|---|---|---|---|---|---|---|
| ⊿CV=1 (hard) | ⊿CV=2 (normal) | ⊿CV=6 (easy) | |||||
| Type S | 15 × 65 | approx. 8 | 7.5 | 5-10 | 0.05 | 0.15 | 0.3 |
| Type M | 20 × 60 | approx. 13 | 15 | 10-20 | 0.1 | 0.3 | 0.6 |
| Type L | 27 × 100 | approx. 35 | 40 | 20-40 | 0.3 | 0.8 | 1.6 |
| Type 2L | 27 × 140 | approx. 50 | 60 | 20-40 | 0.4 | 1.2 | 2.4 |
| Type 3L | 46 × 110 | approx. 115 | 145 | 40-80 | 1.0 | 3.0 | 6.0 |
| Type 4L | 46 × 220 | approx. 230 | 290 | 40-80 | 2.0 | 6.0 | 12.0 |
⊿CV: Difference in retention force of the compound
⊿CV=2 or more will improve the efficiency of fractionation and purification.
Example of use
Comparison of load
*Presep® Silica Gel (HC-N) achieves approximately twice the fractionation compared with FUJIFILM Wako’s existing products.
Presep® (Luer Lock) Silica Gel (SP)
Presep® (Luer Lock) Silica Gel (SP) uses a high-quality spherical silica gel and can perform separation and purification with excellent reproducibility, compared with crushed silica gel. TLC plates (Silica Gel 40F254 TLC Plate-Wako) are recommended for the examination of fractionation conditions.
Features
- Packing of high-quality spherical silica gel
- Excellent reproducibility
-
Example of confirmation of reproducibility
*Comparison of separation among three Type L columns (small inter-lot difference)
-
• Example of separation
*Comparison of column size for Type M, L, and 3L (high resolution is possible regardless of size)
Presep® (Luer Lock) Silica Gel
Presep® (Luer Lock) Silica Gel is a column using high-quality crushed silica gel. It can perform efficient separation and purification. It has a high resolution because it is packed with silica gel of 40 μm particle size. TLC plates (Silica Gel 40F254 TLC Plate-Wako) are recommended for the examination of fractionation conditions.
Features
- Excellent cost performance
- Packed with high-quality silica gel
Example of separation
Presep® (Luer Lock) NH2 (HC) / Presep® (Luer Lock) NH2
Presep® (Luer Lock) NH2 (HC) is a column packed with spherical silica gel and Presep® (Luer Lock) NH2 is a column packed with crushed aminopropyl silica gel, respectively. They can perform separation and purification in normal-phase, reversed-phase, and ion exchange modes.
In addition, they are suitable for separation and purification of strongly basic compounds, and tailing compounds in the normal silica gel column can also be separated and purified well.
Features
- Packing of NH2-modified silica gel with alkalinity
- Strongly basic compounds can be separated and purified
- They can perform separation and purification in normal-phase, reversed-phase, and ion exchange modes
Features of aminopropyl silica gel and comparison with normal silica gel
Comparison of separation of strongly basic compounds
Presep® NH2 (HC) column size and reference value of the recommended flow rate
| Syringe | Size (mm×mm) | Filler Amount (g) | Column Volume (mL) | Recommended Flow Rate (mL/min.) |
|---|---|---|---|---|
| Type S | 15 x 65 | approx. 7 | 7 | 5-10 |
| Type M | 20 x 60 | approx. 14 | 14 | 10-20 |
| Type L | 27 x 100 | approx. 34 | 37 | 20-40 |
| Type 2L | 27 x 140 | approx. 50 | 56 | 20-40 |
| Type 3L | 46 x 10 | approx. 110 | 123 | 40-80 |
| Type 4L | 46 x 220 | approx. 220 | 254 | 40-80 |
Comparison of load
Correlation with Presep® (Luar Lock) NH2 / NH2 Silica Gel 60F254 Plate-Wako
Product List
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Presep® (Luer Lock) Silica Gel (HC-N)
Presep® (Luer Lock) Silica Gel (SP): Spherical
Presep® (Luer Lock) Silica Gel: Crushed
Presep® (Luer Lock) NH2 (HC) / Presep® (Luer Lock) NH2
For research use or further manufacturing use only. Not for use in diagnostic procedures.
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