For the detection of phosphorylated proteins by Western blot

Phos-tag Biotin Probe


Phos-tag Biotin is a biotinylated Phos-tag that can be used for the detection of phosphorylated proteins by Western blot.


  • Able to detect phosphorylated proteins with high sensitivity.
  • Phos-tag binding is not dependent on the composition of amino acids.
  • The procedure is similar to conventional Western blot.

Analysis image

Western blot analysis image by Phos-tag Biotin



  1. "Phosphate-binding tag: A new tool to visualize phosphorylated proteins", E. Kinoshita, E. Kinoshita-Kikuta, K. Takiyama, and T. Koike, Mol. Cell. Proteomics, 2006, 5, 749-757.
  2. "Detection and Quantification of On-Chip Phosphorylated Peptides by Surface Plasmon Resonance Imaging Techniques Using a Phosphate Capture Molecule", K. Inamori, M. Kyo, Y. Nishiya, Y. Inoue, T. Sonoda, E. Kinoshita, T. Koike, and Y. Katayama, Anal. Chem., 2006, 77, 3979-3985.
  3. "Detection of phosphorylated proteins using Phos-tag", Chemiluminescent detection using biotinylated Phos-tag. E. Kinoshita, E. Kikuta-Kinoshita, T. Koide. Experimental Medicine, 2006, 24, 2523-2529.
  4. "Identification on Membrane and Characterization of Phosphoproteins Using an Alkoxide-Bridged Dinuclear Metal Complex as a Phosphate-Binding Tag Molecule", T. Nakanishi, E. Ando, M. Furuta, E. Kinoshita, E. Kikuta-Kinoshita, T. Koike, S. Tsunasawa, and O. Nishimura, J. Biomol. Tech., 2007, 18, 278-286.

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Powder type

Solution type (High Sensitivity)

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