Glucose 6-phosphate Dehydrogenase
Glucose 6-phosphate dehydrogenase (G-6-PDH) is an enzyme in the pentose phosphate cycle, one of the pathways for glucose metabolism. This enzyme catalyzes the following reaction:
G-6-PDH is used for the quantitation of ATP, glucose, and creatine kinase. This product is recombinant glucose 6-phosphate dehydrogenase derived from yeast and Leuconostoc sp.
Product Overview
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G-6-PDH, Leuconostoc sp., recombinant
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G-6-PDH, yeast, recombinant
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| Appearance | White crystals or powder | White crystals, powder or mass |
| Host | E. coli | E. coli |
| Activity | Indicated on the label (initial production lot: 640 units/mg) |
Indicated on the label (initial production lot: 140 units/mg) |
| Definition of unit | The amount of enzyme producing 1 μmol of NADH per minute at pH 7.8 and 30°C, using D-glucose 6-phosphate as a substrate, is defined as one unit. | The amount of enzyme producing 1 μmol of NADPH per minute at pH 8.5 and 25°C, using D-glucose 6-phosphate as a substrate, is defined as one unit. |
| pH stability | 5.0～9.0（25℃, 1 week） | 5.5～6.5（25℃, 1 week） |
| Optimal pH | 7.5 | 8.0～8.5 |
| Temperature stability | ≤ 37℃（pH 7.8, 10 minutes） | ≤ 40℃（pH 7.5, 10 minutes） |
| Optimal temperature | 45℃ | 50℃ |
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For research use or further manufacturing use only. Not for use in diagnostic procedures.
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