C-Peptide

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C-peptide (mouse, rat) ELISA kit

C-peptide is produced simultaneously to insulin. They are included in the insulin precursor - proinsulin. In proinsulin, C-peptide is located as if connecting insulin A and B chains. So, it is called "c(onnecting) peptide". C-peptide is released into the blood in approximately equimolar to insulin by the processing of proinsulin, a precursor of insulin. By the measurement of C-peptide concentration in the blood, it is possible to know the insulin secretory potency of pancreatic β cells in the case during insulin administration or insulin antibodies existing.
LBIS C-peptide Mouse or Rat is a sandwich ELISA system for quantitative measurement of mouse or Rat C-peptide specimen. This is intended for research use only.

Features

  • Measurement range 46.9~3000 pg/mL
  • Measurable in a short period (5 hours)
  • A minute quantity of sample is measurable (10 μL with a standard method of operating)
  • All reagents are solution types and are ready to use
  • High measurement accuracy and reproducibility

Kit Components

1) Antibody-coated plate (8 x 12) wells/plate 1 plate
2) Standard C-peptide solution (6,000 pg/mL) 500 μL
3) Buffer solution 60 mL
4) Biotinylated anti C-peptide Antibody 100 μL
5) HRP-conjugated streptavidin 100 μL
6) TMB Solution 12 mL
7) Stop Solution 12 mL
8) Wash Solution (x 10) 100 mL
9) Plate Seal 4 seals

Cross-reactivity

  • Cross-reactivity of mouse C-peptide ELISA kit

    Sample Substance Cross reaction (%)
    Mouse C-peptide1 100
    C-peptide2 100
    Insulin Less than sensitivity
    Proinsulin Less than sensitivity
    Rat C-peptide 89
    Insulin Less than sensitivity
    Proinsulin Less than sensitivity
    Human C-peptide 85
    Insulin Less than sensitivity
    Proinsulin Less than sensitivity
    Porcine Insulin Less than sensitivity
    Bovine Insulin Less than sensitivity
  • Cross-reactivity of rat C-peptide ELISA kit

    Sample Substance Cross reaction (%)
    Rat C-peptide 100
    Insulin Less than sensitivity
    Proinsulin Less than sensitivity
    Mouse C-peptide 92
    Insulin Less than sensitivity
    Proinsulin Less than sensitivity
    Human C-peptide 90
    Insulin Less than sensitivity
    Proinsulin Less than sensitivity
    Porcine Insulin Less than sensitivity
    Bovine Insulin Less than sensitivtiy

Assay Procedure

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* Guideline of washing volume: 300 μL/well
**Guideline of shaking: 600-1200 rpm for 10 seconds × 3 times

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