Albumin

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FUJIFILM Wako offers ELISA kits for various animal species and Turbidimetric Immuno Assay (TIA) reagents for automated measurement.

Albumin (Mouse,Rat) ELISA Kit

LBIS Mouse/Rat Albumin ELISA Kit is a sandwich ELISA system for quantitative measurement of mouse/Rat albumin. This is intended for research use only.

Features

  • Measurable in a short period (Total reaction time: 2 hours and 20 minutes)
  • A minute quantity of sample is measurable (5 μL with a standard method of operating)
  • Eco-friendly preservatives are used
  • All reagents are solution types and are ready to use
  • High measurement accuracy and reproducibility

Sample

Serum, Plasma or urine

  • Heparin is recommended to be used for collection of plasma.
  • Samples are diluted within the range of measurement with the accompanying buffering solution.
  • Rough indications of dilution are 10,000 to 50,000 times for a serum or plasma sample and 50 to 100 times for a urine sample.

Accuracy test

Precision of assay : Mean CV<10%
Reproducibility : Mean CV<10%

Specificity

The antibodies used in this kit are specific to albumin. Cross-reactivity of the kit is shown below.

  • Albumin Mouse ELISA Kit

    ※Cross reaction at 10000 ng/mL (Except FCS)

    Sample Cross reaction
    Rat Albumin Less than 5%
    Human Albumin N.D.
    Porcine Albumin N.D.
    Bovine Albumin N.D.
    10 % FCS N.D.
  • Albumin Rat ELISA Kit

    ※Cross reaction at 10000 ng/mL (Except FCS)

    Sample Cross Reaction
    Mouse Albumin Less than 5%
    Human Albumin N.D.
    Porcine Albumin N.D.
    Bovine Albumin N.D.
    10 % FCS N.D.

Standard curve

  • Mouse Albumin assay standard curve

    00840_img01.png

  • Rat Albumin assay standard curve

    00840_img02.png

Kit Components

1) Anti-albumin coated plate(8 x 12) 1 plate
2) Standard albumin mouse or rat(10 μg/mL) 1 vial
3) Buffer solution 60 mL
4) HRP-labeled anti-albumin antibody 100 μL
5) TMB Solution 12 mL
6) Stop Solution 12 mL
7) Wash stock Solution (x 10) 100 mL
8) Plate Seal 3 seals

References

Albumin Mouse ELISA Kit

  1. Mizukami, K. et al.: Naunyn. Schmiedebergs. Arch. Pharmacol., 391, 1319(2018)
  2. Rhee, H. et al.: Kidney. Res. Clin. Pract., 37, 222(2018)
  3. Ishikawa, H. et al.: Biosci. Biotechnol. Biochem., 82, 515(2018)

Albumin Rat ELISA Kit

  1. Kanno, M. et al.: Nephrology., 23, 1046(2018)
  2. Ishibashi, Y. et al.: Horm. Metab., 48, 613(2016)
  3. Takeshige, Y. et al.: Hypertens., 39, 415(2016)

Turbidimetric Immuno Assay Kit (TIA Kit)

Features

  • Measurement range Mouse/rat 6.17 to 500 μg/mL
  • Samples are urine or serum
  • Special antibodies for each species (monkey/mouse/rat albumin) are used for a specific reaction
  • Measurable in a short period (10 minutes) with a generalpurpose biochemical autoanalyzer
  • Wide measurement range with superior reproducibility
  • It cannot be affected by manipulation because it is measured with an autoanalyzer
  • Parameters for each autoanalyzer made by domestic manufacturers are being prepared (except a part of them)

Accuracy test

Mouse, rat in common

Precision of assay : Mean CV<7.5%
Reproducibility : Mean CV<7.5%

Kit Components

1) Reagent 1 (Buffer) 18.5 mL
2) Reagent 2 (Anti-mouse or rat albumin solution) 6.6 mL
3) Standard mouse or rat albumin* 2.0 mL
4) Diluent solution for standard albumin 4.0 mL

*Concentration of standard mouse or rat albumin is indicated on its bottle label.

Assay procedure

For Hitachi 7180 autoanalyzer
*Note that operation of autoanalyzer is different depending on the type of machines

00840_img03.png

References

LBIS Mouse Urinary Albumin Assay Kit

  1. Haku, S. et al.: Biomed. Res. Int., 2018, 2817045(2018)
  2. Iwashita, Y. et al.: Am. J. Physiol. Renal. Physiol., 310, F1206(2016)
  3. Zhu, K. et al.: Free. Radic. Biol. Med., 83, 21(2015)

LBIS Rat Urinary Albumin Assay Kit

  1. Tatsuyori, M. et al.: Eur. J. Pharmacol., 756, 85(2015)
  2. Ruixia, M. et al.: Int. J. Clin. Exp. Pathol., 8, 14063(2015)
  3. Sakai, M. et al.: Eur. J. Pharmacol., 761, 109(2015)

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Albumin (Mouse,Rat) ELISA Kit

TIA reagent for automatic measuring instrument

Product content may differ from the actual image due to minor specification changes etc.

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