Biostir Atopic Dermatitis-Inducing Reagent

Biostir AD is ointment including mite allergens derived from Dermatophagoides farinae. It induces atopic dermatitis in NC/Nga mice 1 to 2 weeks after application twice a week for a total of 6 times.

Features

• The extract of house dust mites, which are relevant to atopic dermatitis, is one of the ingredients.
• Biostir AD is able to induce atopic dermatitis-like skin lesion in NC/Nga mice.
• The efficacy of both tacrolimus ointment and corticosteroid ointment is observed in this model.
• Biostir AD is able to cause dermatitis to NC/Nga mice with a high probability
• Biostir AD will save time and effort until the onset of dermatitis observed when compared to the repeated applications of mite allergen suspension
• There are no products with a similar concept

Induction method

How to use "Biostir AD"

After barrier disruption with 4 % sodium dodecyl sulfate, apply 100 mg of Biostir AD on the shaved dorsal skin and both surfaces of each ear two times a week. Repeat this procedure for 3 weeks. The application is done 6 times in total.
Protocol for Biostir AD ointment

Scoring examples

1) Redness/bleeding (The back is observed for redness and bleeding symptoms.)
　0: [No symptom] Neither redness nor bleeding symptom on the back
　1: [Mild] Local redness on the back without bleeding associated with continuous scratch
　2: [Moderate] Scattered redness on the back without bleeding associated with continuous scratch
　3: [Severe] Extensive redness or bleeding associated with continuous scratch on the back

2) Crust formation/dryness (The back is observed for crust formation and dryness symptoms.)
　0: [No symptom] Neither crust formation nor dryness symptom on the back
　1: [Mild] Local crust or dryness symptoms on the back, with slightly pale skin and slight keratinous detachment
　2: [Moderate] Scattered crust or dryness symptoms, or obvious keratinous detachment on the back
　3: [Severe] Extensive crust or obvious keratinous detachment on the back

3) Edema (The auricle is qualitatively observed for edema.)
　0: [No symptom] No thickening of either auricle
　1: [Mild] Slight thickening of either auricle
　2: [Moderate] Obvious thickening and tightness of both auricles
　3: [Severe] Obvious thickening, tightness, and curvature of both auricles with hardness when touched with a finger

4) Scratch/tissue loss (The auricle is qualitatively observed for scratch and tissue loss symptoms.)
　0: [No symptom] Neither scratch nor tissue loss symptom in the auricle
　1: [Mild] Non-continuous scratch in the auricle without tissue loss
　2: [Moderate] Small continuous scratch in the auricle without tissue loss
　3: [Severe] Continuous scratch and tissue loss in the auricle