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  4. Antigen Retrieval Reagents
  5. ImmunoSaver
Antigen retrieval reagent

ImmunoSaver

ImmunoSaver is an antigen retrieval reagent for formalin-fixed tissues. This product is used to restore antigenicity that was reduced by formalin fixation. As a result, the quality of immunostaining is improved. While various conditions have to be examined in conventional methods, this product can retrieve a wide range of antigens under the same conditions.

Features

  • It restores the antigenicity of formalin-fixed tissues.
  • It improves the quality of immunostaining.
  • It retrieves a wide range of antigens under the same conditions.

Composition

10% sodium citraconate solution

Usage

  1. Dilute ImmunoSaver by a factor of 200 with distilled or deionized water, and heat to 98°C.
  2. Place deparaffinized tissue sections in the diluted solution, and incubate at 98°C for 45 minutes.
  3. Wash the tissue sections with PBS three times for 5 minutes each, and proceed to immunostaining.

Example of staining

  1. Treat formalin-fixed human pancreatic paraffin sections with ImmunoSaver.
  2. Wash the paraffin sections once with PBST (PBS containing Triton X-100 at a concentration of 0.2%) and twice with PBS.
  3. Block at room temperature for 1 hour.
  4. Rinse the sections gently with PBS and react them with the primary antibody diluted in PBST at 4°C overnight. (rabbit anti SNAP-25 1/8,000)
  5. Wash the sections once with PBST and twice with PBS.
  6. React the sections with the biotin-labeled secondary antibody at room temperature for 1 hour.
  7. Wash the sections once with PBST and twice with PBS.
  8. React the sections with peroxidase-labeled avidin (1/400, diluted in PBS) at room temperature for 1 hour.
  9. Wash the sections once with PBST and twice with PBS.
  10. Use DAB as substrate for chromogenic reaction.
  11. Wash the sections with distilled water for 5 minutes, three times.
  12. Stain the sections with Mayer's acidic hemalum solution for approximately 1 to 3 minutes.
  13. Wash the sections with running water, rinse with distilled water two or three times, and discriminate color with 70% ethanol.
  14. Clear and mount the sections.

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For research use or further manufacturing use only. Not for use in diagnostic procedures.

Product content may differ from the actual image due to minor specification changes etc.

If the revision of product standards and packaging standards has been made, there is a case where the actual product specifications and images are different.

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