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Xylene substitutes

PathoClean

Data by courtesy of Kasumigaura Medical Center, National Hospital Organization

In Japan, with the partial revision of Order for Enforcement of the Industrial Safety and Health Act, xylene is now subject to the "Ordinance on Prevention of Organic Solvent Poisoning" and women cannot work in an environment that contains more than a controlled concentration of 50 ppm.

Use this product to replace xylene.

Examples of comparative staining with xylene and PathoClean (Human)

H&E staining (stomach)

  • Left: Xylene
  • Right:PathoClean

H&E staining (fallopian tube)

  • Left: Xylene
  • Right:PathoClean

Elastica van Gieson staining (lung)

  • Left: Xylene
  • Right:PathoClean

Periodic acid-Schiff staining (kidney)

  • Left: Xylene
  • Right:PathoClean

Masson's trichrome staining (liver)

  • Left: Xylene
  • Right:PathoClean

Data by courtesy of Kasumigaura Medical Center, National Hospital Organization

Examples of comparative staining with xylene and PathoClean (Mouse)

Immunohistochemical staining-PCNA staining

Material: Mouse breast cancer, formalin-fixed

  • Left: Xylene
  • Right:PathoClean

H&E staining

Material: Mouse breast cancer, formalin-fixed

  • Left: Xylene
  • Right:PathoClean

Data by courtesy of Dr. Masaaki Shibata, Department of Anatomy and Cell Biology , Division of Life Sciences, Osaka Medical College

Features

Xylene or other compounds that are compatible with alcohol and paraffin are commonly used as an intermediate agent. PathoClean can be used instead of these compounds in the process of deparaffinization and permeation.

*We recommend PathoMount (Product Number 164-28492, 168-28495) or UI Sol (UI Kasei) as a mounting agent for PathoClean. Please be aware that white turbidity may occur when Marinol (Muto Pure Chemicals Co., Ltd.) is used.

Examples of H&E staining protocol

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