Ordering
A marker for airway and alveolar progenitor cells

Anti CPM, Monoclonal Antibody (WK)

Carboxypeptidase M (CPM) is an enzyme present on the surface of the cell membrane that catalyzes cleavage of C-terminal arginine and lysine residues from polypeptides and proteins.
Recent studies have reported that CPM can be used as a marker for airway, alveolar and hepatic progenitor cells.

Product outline

  • Formulation: Phosphate buffer containing 50w/v% glycerol and 0.05w/v% sodium azide
  • Concentration: 1.0 mg/mL (initial measured value)
  • Subclass: Mouse IgG2b
  • Applications
    Flow cytometry 1:100~200
    Immunofluorescence 1:100~1,000
    Western blot 1:1,000

Example of Use

Human iPS cells were differentiated into lung progenitor cells. The differentiated cells were dissociated, stained with this newly developed CPM antibody, and sorted with flow cytometry. Cell populations that highly expressed CPM were consequently found to be suitable for differentiation into alveolar and airway epithelial cells.

00574_img01.jpg

< Data provided by Shimpei Goto, Graduate School of Medicine, Kyoto University >

References

  • Yamamoto, Y., Gotoh, S., Korogi, Y., Seki, M., Konishi, S., Ikeo, S., Sone, N., Nagasaki, T., Matsumoto, H., Muro, S., Ito, I., Hirai, T., Kohno, T., Suzuki, Y. and Mishima, M.: Nat. Methods,14, 1097 (2017).
  • Hawkins, F., Kramer, P., Jacob, A., Driver, I., Thomas, DC., McCauley, KB., Skvir, N., Crane, AM., Kurmann, AA., Hollenberg, AN., Nguyen, S., Wong, BG., Khalil, AS., Huang, SX., Guttentag, S., Rock, JR., Shannon, JM., Davis, BR. and Kotton, DN.: J. Clin. Invest., 127, 2277 (2017).
  • Konishi, S., Gotoh, S., Tateishi, K., Yamamoto, Y., Korogi, Y., Nagasaki, T., Matsumoto, H., Muro, S., Hirai, T., Ito, I., Tsukita, S. and Mishima, M.: Stem Cell Reports, 6, 18 (2016).
  • Kido, T., Koui, Y., Suzuki, K., Kobayashi, A., Miura, Y., Chem, EY., Tanaka, M. and Miyajima, A.: Stem Cell Reports, 5, 508 (2015).
  • Gotoh, S., Ito, I., Nagasaki, T., Yamamoto, Y., Konishi, S., Korogi, Y., Matsumoto, H., Muro, S., Hirai, T., Funato, M., Mae, S., Toyoda, T., Sato-Otsubo, A., Ogawa, S., Osafune, K. and Mishima, M.: Stem Cell Reports, 3, 394 (2014).

Product List

  • Open All
  • Close All

Product content may differ from the actual image due to minor specification changes etc.

877-714-1920

Hours of Operation: 8:00 - 17:00 (EST)For other hours than the above, please contact us via the inquiry form.