Anionic Surfactant (LAS) Analysis
Chemical Name: Sodium Dodecylbenzene Sulfonate
Molecular Formula: C16H25NaO3S
Anionic surfactants which are contaminated from factory wastewater and domestic sewage are considered to be an important indicator of water pollution because they cause foaming when present in water. Linear alkylbenzene sulfonic acid (LAS), a kind of anionic surfactant, is used in the largest amount among synthetic detergents. For anionic surfactants, standard values are set for Drinking Water Quality Standards and Environmental Quality Standards for Water Pollution in Japan.
Fujifilm Wako provides various standard solutions for anionic surfactants that are subject to measurement in Japanese Drinking Water Quality Standards, internal standard solutions that can be used for LC/MS, and HPLC columns.
Standard Solution
Five types of anionic surfactants that are subject to measurement in Japanese Drinking Water Quality Standards (sodium decylbenzenesulfonate, sodium undecylbenzenesulfonate, sodium dodecylbenzenesulfonate, sodium tridecylbenzenesulfonate, and sodium tetradecylbenzenesulfonate, their phenyl group is bonded to a carbon other than the terminal of a linear-chain alkyl group). You can use them for calibration curve creation.
In addition, Fujifilm Wako also offers a mixture standard solution in which the above five anionic surfactants are mixed at 1 mg/mL each (methanol solution).
Mixture standard solution
LAS (C10-C14), in which a phenyl group is bonded to carbons other than the terminal of the linear alkyl chain, are mixed. And the concentration values of each LAS are specified. It can be used for the notification test method of the Japanese Drinking Water Quality Standards.
| Code No. | Product Name | Grade | Volume |
|---|---|---|---|
| 013-20131 | Anionic Surfactants Mixture Standard Solution (each 1 mg/mL Methanol Solution) | for Water Analysis | 1 mL x 5 A |
- [HPLC Chromatogram]
- [Composition information]
Peak No. Anionic Surfactant C10 Sodium Decylbenzenesulfonate C11 Sodium Undecylbenzenesulfonate C12 Sodium Dodecylbenzenesulfonate C13 Sodium Tridecylbenzenesulfonate C14 Sodium Tetradecylbenzenesulfonate - [HPLC conditions]
Column Wakopak® Navi C18-5 4.6 mm x 250 mm Column temperature 40℃ Eluent 0.1 mol/L NaClO4 in CH3CN/H2O= 65/35 Flow rate 1.0 mL/min. Detection UV 225 nm
Single Standard Solution
| Code No. | Product Name | Grade | Volume |
|---|---|---|---|
| 195-13111 | Sodium Decylbenzenesulfonate Standard Solution (1 mg/mL Methanol Solution) | for Water Analysis | 1 mLx5 A |
| 192-13121 | Sodium Undecylbenzenesulfonate Standard Solution (1 mg/mL Methanol Solution) | for Water Analysis | 1 mLx5 A |
| 199-13131 | Sodium Dodecylbenzenesulfonate Standard Solution (1 mg/mL Methanol Solution) | for Water Analysis | 1 mLx5 A |
| 196-13141 | Sodium Tridecylbenzenesulfonate Standard Solution (1 mg/mL Methanol Solution) | for Water Analysis | 1 mLx5 A |
| 193-13151 | Sodium Tetradecylbenzenesulfonate Standard Solution (1 mg/mL Methanol Solution) | for Water Analysis | 1 mLx5 A |
Internal Standard Solution
Fujifilm Wako provides internal standard solutions that can be used for LC/MS analysis of anionic surfactants. Sodium 4-Dodecylbenzenesulfonate-13C6 Standard Solution has physical properties close to those of C12-LAS, which is subject to various regulations in Japan, and can be used as an internal standard.
Internal standard solution
| Code No. | Product Name | Grade | Volume |
|---|---|---|---|
| 195-17131 | Sodium p-n-Octylbenzenesulfonate Standard Solution (1 mg/mL Methanol Solution) | for Water Analysis | 1 mL |
| 191-19171 | Sodium 4-Dodecylbenzenesulfonate-13C6 Standard Solution (10 μg/mL Methanol Solution) | for Water Analysis | 1 mL x 5 A |
Solid Phase Extraction Column
Recovery test of sodium alkylbenzenesulfonate (C10-C14) in water samples using Presep®-C C18 and Presep®-Agri
(Compliant with the Japanese Drinking Water Quality Standards (enforced on April 1, 2004))
HPLC Column
Wakopak® Wakosil-Ⅱ
LC/MS/MS analysis example of LAS (C10-C14) using the Japanese Drinking Water Quality Standards notification method (HPLC column: Wakopak® Wakosil-Ⅱ 3C8RS)
LC/MS/MS Chromatogram
| LAS | Monitoring Ions (m/z) | Mode | LAS | Monitoring Ions (m/z) | Mode | LAS | Monitoring Ions (m/z) | Mode | |||
|---|---|---|---|---|---|---|---|---|---|---|---|
| Precursor | Products | Precursor | Products | Precursor | Products | ||||||
| C10-LAS | 297 | 183 | - | C12-LAS | 325 | 183 | - | C14-LAS | 353 | 183 | - |
| C11-LAS | 311 | 183 | - | C13-LAS | 339 | 183 | - | 13C12-LAS | 331 | 176 | - |
Analysis conditions
Shimadzu LC/MS-8040
| Column： | Wakopak® Wakosil-Ⅱ 3C18RS 2.0×150 mm | ||||
| Column temperature： | 40℃ | ||||
| Eluent： | A) H2O, B) CH3CN | ||||
| Gradient： |
|
||||
| Flow rate： | 0.3 mL/min. |
Ionization: ESI
Mode: MRM
LC/MS/MS analysis example of LAS (C10-C14) using the Japanese Drinking Water Quality Standards notification method (HPLC column: Wakopak® Wakosil-Ⅱ 3C18HG)
LC/MS/MS Chromatogram
| LAS | Monitoring Ions (m/z) | Mode | LAS | Monitoring Ions (m/z) | Mode | LAS | Monitoring Ions (m/z) | Mode | |||
|---|---|---|---|---|---|---|---|---|---|---|---|
| Precursor | Products | Precursor | Products | Precursor | Products | ||||||
| C10-LAS | 297 | 183 | - | C12-LAS | 325 | 183 | - | C14-LAS | 353 | 183 | - |
| C11-LAS | 311 | 183 | - | C13-LAS | 339 | 183 | - | 13C12-LAS | 331 | 176 | - |
Analysis conditions
Shimadzu LC/MS-8040
| Column： | Wakopak® Wakosil-Ⅱ 3C18HG 2.0×150 mm | ||||
| Column temperature： | 40℃ | ||||
| Eluent： | A) 0.1 vol% HCOOH in H2O, B) 0.1 vol% HCOOH in CH3CN | ||||
| Gradient： |
|
||||
| Flow rate： | 0.3 mL/min. |
Ionization: ESI
Mode: MRM
Wakopak® Wakosil® AS-Aqua
Linear alkylbenzene sulfonic acid (LAS) exists as a mixture of isomers, and many peaks are detected when use ODS columns.
Wakopak® Wakosil® AS-Aqua separates isomers as a single peak for each carbon length of LAS, which improves detection sensitivity, simplifies quantitative calculations, and shortens analysis time. Also, since the linear alkylbenzene sulfonic acids (LAS) and branched alkylbenzene sulfonic acids are separated, they can be distinguished. Furthermore, there is no significant difference in the measured values compared to a general ODS column, and more accurate measurements can be performed even in the low-concentration condition.
Comparison of ODS Columns and Wakopak® Wakosil® AS-Aqua for Anionic Surfactant Analysis
[HPLC]
| Eluent | CH3CN/H2O=65/35 (v/v)+ 12.3 g/1,000 mL NaClO4 added |
|---|---|
| Flow rate | 1.0 mL/min. (Wakopak® Navi C18-5), 0.7 min. (Wakopak® Wakosil® AS-Aqua) |
| Detection | FL Ex. 221 nm, Em. 284 nm |
| Injection volume | 10 µL |
| Sample | Anionic Surfactants Mixture Standard Solution (C10-C14 each 10 mg/mL Methanol Solution) |
Column: Wakopak® Navi C18-5 4.6 mmx250 mm (ODS column)
Column: Wakopak® Wakosil® AS-Aqua*
* Wakopak® Wakosil® AS-Aqua can recognize and separate both linear and branched alkylbenzene sulfonic acids.
Separation of anionic surfactants mixture standard solution and toluene
Depending on the pretreatment method, toluene may be mixed in the extracted sample.
When using Wakopak® Wakosil® AS-Aqua, toluene and anionic surfactants are eluted separately, allowing analysis without interference from the toluene peak.
-
[HPLC]
Column : Wakopak® Wakosil® AS-Aqua 4.6 mm x 250 mm Column temperature : 40℃ Eluent : CH3CN/H2O = 65/35 (v/v) + 12.3 g/1,000 mL NaClO4 added Flow rate : 0.7 mL/min. Detection : (Upper) FL Ex. 221 nm. Em. 284 nm (Lower) UV 225 nm Injection volume : 10 μL Sample : Anionic Surfactants Mixture Standard Solution
(C10-C14 each 10 mg/mL Methanol Solution)+Toluene (1 μL/mL) added
Product List
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Mixture Standard Solution
Single Standard Solution
Internal Standard Solution
HPLC Column
Dedicated Eluent for Wakopak Wakosil AS-Aqua
Solid Phase Extraction Column
Measurement Kit
For research use or further manufacturing use only. Not for use in diagnostic procedures.
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