LBIS™ Human TNF-α ELISA Kit

About samples

What anticoagulants should I use?

We recommend using EDTA or heparin as anticoagulant.

How should I store and prepare the samples?

Samples should be immediately assayed or stored below -35℃ until assay. Before starting assay, shake thawed samples sufficiently. Do not repeat freeze-and-thaw cycles. Dilution of a sample should be made in a PP or PE test tube using the provided buffer prior to adding them to wells.

About kit usage

What instruments, and equipment are required for the assay using this kit?

The instruments and equipment required for the use of this kit are listed below;

• Deionized water (or distilled water)
• Test tubes for preparation of standard solution series
• Glassware for dilution of Wash Solution (10x) (a graduated cylinder, a bottle)
• Pipettes (disposable tip type). One should be able to deliver 50 μL precisely, and another for 100 – 1,000 μL
• Syringe-type repeating dispenser like Eppendorf multipette plus which can dispense 100 μL
• Paper towel to remove washing buffer remaining in wells
• A vortex-type mixer
• A shaker for 96 well-plate (600 – 1,200 rpm)
• An automatic washer for 96 well-plate (if available), or a wash bottle with a jet nozzle
• A 96 well-plate reader (450 ± 10 nm, 620 nm :600 - 650 nm)
• Software for data analysis

How should I use the human TNF-α standard?

The amount of purified water to be added varies by lot. Please reconstitute the human TNF-α standard with the amount of purified water specified in the “Reconstitution of standard” section on this product page to prepare the original standard solution (5 ng/mL). Then, dilute the solution using the kit-provided buffer that has been equilibrated to room temperature.

What are the storage conditions for each reagent?

[Buffer & TMB Solution]
Use only volume you need for your assay. Remaining reagents should be stored at 2-10℃ closing the cap tightly. Once opened, we recommend using as soon as possible to avoid influence by environmental condition.
[Stop Solution]
Close the cap tightly and store at 2-10℃.
[Human TNF-α Standard & Biotin-conjugated Antibody]
The reconstituted standard solution (original standard solution) should be used within 2 weeks if stored in a refrigerator. Dispose the remaining diluted standard solutions after use.
[Peroxidase-conjugated Streptavidin Solution]
Remaining working solution (already diluted) should be disposed. The rest of the undiluted solution (unused): close the cap tightly and store at 2-10℃. Once opened, we recommend using as soon as possible to avoid influence by environmental condition.

Prepare reagent solutions in appropriate volume for your assay.