LBIS™ Mouse MCP-1 (CCL2) ELISA Kit
- for Immunochemistry
- Manufacturer :
- FUJIFILM Wako Pure Chemical Corporation
- Storage Condition :
- Keep at 2-10 degrees C.
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96Tests
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In stock in Japan |
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Kit component
96 tests
| Antibody-coated Plate | 96 wells/1 plate |
|---|---|
| Mouse MCP-1 (CCL2) Standard | 1 bottle |
| Buffer | 60 mL/1 bottle |
| Biotin-conjugated Antibody | 1 bottle |
| Peroxidase-conjugated Streptavidin Solution | 100 μL/1 bottle |
| TMB Solution | 12 mL/1 bottle |
| Stop Solution | 12 mL/1 bottle |
| Wash Solution (10x) | 100 mL/1 bottle |
| Plate Seal | 4 sheets |
Product Overview
MCP-1 (CCL2) is a member of the CC chemokine family and exhibits chemotactic activity toward monocytes and macrophages. Because MCP-1 and its receptor recruit these cells to sites of inflammation--thereby sustaining inflammatory responses--they are implicated in metabolic syndrome and cardiovascular diseases.
LBIS™ Mouse MCP-1 (CCL2) ELISA Kit is a sandwich ELISA kit designed for the quantitative measurement of mouse MCP-1 (CCL2). This kit enables highly sensitive quantification of MCP-1 (CCL2) in biological samples. The standard included in the kit is a recombinant protein produced in Escherichia coli.
Kit Performance
| Assay target | MCP-1 (CCL2) |
|---|---|
| Calibration curve range | 3.85 – 500 pg/mL |
| Analysis sample | Mouse Serum/Plasma (EDTA/heparin)/Urine/ Culture medium (D-MEM/RPMI-1640 etc.)* |
| Sample amount | 10 μL (5x dilution) |
| Measurement duration | Approx 3 hours and 50 minutes |
| Wavelength | Primary wavelength : 450 nm Reference wavelength : 620 nm |
*It is tested by using the samples that standard substances have been added to the culture medium.
Measurement availability depends on the culture medium.
When using cell culture supernatant as a sample, please make sure to perform recovery test and dilution linearity test on the culture medium before use.
Measurement availability depends on the culture medium.
When using cell culture supernatant as a sample, please make sure to perform recovery test and dilution linearity test on the culture medium before use.
Example of Calibration Curve
FAQ
About samples
- What anticoagulants should I use?
- We recommend using EDTA or heparin as anticoagulant.
- How should I store and prepare the samples?
- Samples should be immediately assayed or stored below
-35℃ until assay. Before starting assay, shake thawed samples sufficiently. Do not repeat freeze-and-thaw cycles.
Dilution of a sample should be made in a PP or PE test tube using the provided buffer prior to adding them to wells.
About kit usage
- What instruments, and equipment are required for the assay using this kit?
- The instruments and equipment required for the use of this kit are listed below;
・Deionized water (or distilled water)
・Test tubes for preparation of standard solution series
・Glassware for dilution of Wash Solution (10x) (a graduated cylinder, a bottle)
・Pipettes (disposable tip type). One should be able to deliver 50 μL precisely, and another for 100 – 1,000 μL
・Syringe-type repeating dispenser like Eppendorf multipette plus which can dispense 100 μL
・Paper towel to remove washing buffer remaining in wells
・A vortex-type mixer
・A shaker for 96 well-plate (600 – 1,200 rpm)
・An automatic washer for 96 well-plate (if available), or a wash bottle with a jet nozzle
・A 96 well-plate reader (450 ± 10 nm, 620 nm :600 - 650 nm)
・Software for data analysis
- How should I use the mouse MCP-1 standard?
- The amount of purified water to be added varies by lot. Please reconstitute the mouse MCP-1 standard with the amount of purified water specified in the “Reconstitution of standard” section on this product page to prepare the original standard solution (5,000 pg/mL). Then, dilute the solution using the kit-provided buffer that has been equilibrated to room temperature.
- What are the storage conditions for each reagent?
- Buffer & TMB Solution
Use only volume you need for your assay. Remaining reagents should be stored at 2-10℃ closing the cap tightly. Once opened, we recommend using as soon as possible to avoid influence by environmental condition.
Stop Solution
Close the cap tightly and store at 2-10℃.
Mouse MCP-1 Standard & Biotin-conjugated Antibody
Mouse MCP-1 Standard & Biotin-conjugated Antibody The reconstituted standard solution (original standard solution) should be used within 2 weeks if stored in a refrigerator. Dispose the remaining diluted standard solutions after use.
Peroxidase-conjugated Streptavidin Solution
Remaining working solution (already diluted) should be disposed. The rest of the undiluted solution (unused): close the cap tightly and store at 2-10℃. Once opened, we recommend using as soon as possible to avoid influence by environmental condition.
Remaining working solution (already diluted) should be disposed. The rest of the undiluted solution (unused): close the cap tightly and store at 2-10℃. Once opened, we recommend using as soon as possible to avoid influence by environmental condition.
Overview / Applications
Property
Manufacturer Information
Alias
- 637-54101 (Previous code)
AKMMCP1-011 (Previous code)
For research use or further manufacturing use only. Not for use in diagnostic procedures.
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