LBIS (TM) Human IL-7 ELISA Kit
- for Immunochemistry
- Manufacturer :
- FUJIFILM Wako Pure Chemical Corporation
- Storage Condition :
- Keep at 2-10 degrees C.
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96Tests
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Kit component
96 tests
| Antibody-coated Plate | 96 wells/1 plate |
|---|---|
| Human IL-7 Standard | 1 bottle |
| Buffer | 60 mL/1 bottle |
| Biotin-conjugated Antibody | 1 bottle |
| Peroxidase-conjugated Streptavidin Solution | 150 μL/1 bottle |
| TMB Solution | 12 mL/1 bottle |
| Stop Solution | 12 mL/1 bottle |
| Wash Solution (10x) | 100 mL/1 bottle |
| Plate Seal | 4 sheets |
Product Overview
Human Interleukin-7 (IL-7) is a glycoprotein with a molecular weight of approximately 25,000 Da, composed of 152 amino acid residues, and is an essential cytokine for the adaptive immune system. IL-7 promotes the differentiation of immune cells such as natural killer (NK) cells, B cells, and T cells, as well as host defense. It is involved in chronic infectious diseases, rheumatoid arthritis, asthma, and other conditions.
LBIS™ Human IL-7 ELISA Kit is a sandwich ELISA kit for the quantitative measurement of human IL-7. This kit enables highly sensitive quantification of IL-7 in biological samples. The standard included in the kit is a recombinant protein produced in Escherichia coli.
Kit Performance
| Assay target | IL-7 |
|---|---|
| Calibration curve range | 1.50 - 236 pg/mL |
| Analysis sample | Human Serum/Plasma (EDTA, heparin) |
| Sample amount | 50 μL (2x dilution) |
| Measurement duration | Approx 2 hours and 50 minutes |
| Wavelength | Primary wavelength : 450 nm Reference wavelength : 620 nm |
Example of Calibration Curve
Data
Repeatability (within run precision)
Repeatability was conducted by measuring fivefold assays of human serum samples at two concentrations.
| n＼ID | ID1 (pg/mL) | ID2 (pg/mL) |
|---|---|---|
| 1 | 2.00 | 30.4 |
| 2 | 2.13 | 31.6 |
| 3 | 2.21 | 31.5 |
| 4 | 2.14 | 32.1 |
| 5 | 2.14 | 31.3 |
| mean | 2.12 | 31.4 |
| SD | 0.076 | 0.62 |
| CV(%) | 3.6 | 2.0 |
- [Result]
- The CV (%) of human serum was 2.0-3.6%, indicating good repeatability.
Reproducibility (between run precision)
Reproducibility was conducted by measuring human serum samples at three concentrations each for four days.
| Day＼ID | ID3 (pg/mL) | ID4 (pg/mL) | ID5 (pg/mL) |
|---|---|---|---|
| 1 | 124 | 7.72 | 4.08 |
| 2 | 126 | 7.93 | 3.99 |
| 3 | 129 | 7.86 | 4.18 |
| 4 | 121 | 7.64 | 3.88 |
| mean | 125 | 7.79 | 4.03 |
| SD | 3.4 | 0.13 | 0.13 |
| CV(%) | 2.7 | 1.7 | 3.2 |
- [Result]
- The CV (%) of human serum was 1.7-3.2%, indicating good reproducibility.
Dilution Linearity Test
Human serum and plasma (EDTA/heparin) were serially diluted twofold using the provided buffer and measured in duplicate to evaluate linearity.
Human serum
Human plasma (EDTA)
Human plasma (heparin)
- [Result]
- All measurement results showed good linearity within the measurement range.
Spike and Recovery Test
Standard solutions at three concentrations were added to human serum/plasma (EDTA), then spike recovery tests were conducted, with each measurement performed in duplicate.
Human serum
| Spiked amount (pg/mL) | Measurement value (pg/mL) | Recovery amount (pg/mL) | Recovery (%) |
|---|---|---|---|
| - | 8.37 | - | - |
| 12.5 | 19.9 | 11.5 | 92.0 |
| 25.0 | 31.9 | 23.5 | 94.0 |
| 50.0 | 57.5 | 49.1 | 98.2 |
| 100 | 113 | 105 | 105 |
| mean | 97.3 | ||
Human plasma (EDTA)
| Spiked amount (pg/mL) | Measurement value (pg/mL) | Recovery amount (pg/mL) | Recovery (%) |
|---|---|---|---|
| - | 8.33 | - | - |
| 12.5 | 19.9 | 11.6 | 92.8 |
| 25.0 | 32.4 | 24.1 | 96.4 |
| 50.0 | 57.7 | 49.4 | 98.8 |
| 100 | 109 | 101 | 101 |
| mean | 96.7 | ||
Human plasma (heparin)
| Spiked amount (pg/mL) | Measurement value (pg/mL) | Recovery amount (pg/mL) | Recovery (%) |
|---|---|---|---|
| - | 8.65 | - | - |
| 12.5 | 21.2 | 12.6 | 101 |
| 25.0 | 33.6 | 25.0 | 100 |
| 50.0 | 58.9 | 50.3 | 101 |
| 100 | 113 | 104 | 104 |
| mean | 101 | ||
- [Result]
- Good recovery rates were confirmed.
Example of Sample Measurement
Human serum samples were measured and compared using this kit.
| ID＼n | Measurement value (pg/mL) | mean | SD | CV (%) | |
|---|---|---|---|---|---|
| 1 | 2 | ||||
| 1 | 3.85 | 3.95 | 3.90 | 0.071 | 1.8 |
| 2 | 2.53 | 2.36 | 2.45 | 0.12 | 4.9 |
| 3 | 7.83 | 7.35 | 7.59 | 0.34 | 4.5 |
| 4 | 6.39 | 6.03 | 6.21 | 0.25 | 4.1 |
| 5 | 9.55 | 9.18 | 9.37 | 0.26 | 2.8 |
| 6 | 5.53 | 5.17 | 5.35 | 0.25 | 4.8 |
| 7 | 3.69 | 3.86 | 3.78 | 0.12 | 3.2 |
| 8 | 10.7 | 11.3 | 11.0 | 0.42 | 3.9 |
| 9 | 39.2 | 40.8 | 40.0 | 1.1 | 2.8 |
| 10 | 9.85 | 10.2 | 10.0 | 0.25 | 2.5 |
| 11 | 3.41 | 3.58 | 3.50 | 0.12 | 3.4 |
- [Result]
- This product was able to detect IL-7.
FAQ
About samples
- What anticoagulants should I use?
- We recommend using EDTA or heparin as anticoagulant.
- How should I store and prepare the samples?
- Samples should be immediately assayed or stored below -35℃ until assay. Before starting assay, shake thawed samples sufficiently. Do not repeat freeze-and-thaw cycles. Dilution of a sample should be made in a PP or PE test tube using the provided buffer prior to adding them to wells.
About kit usage
- What instruments, and equipment are required for the assay using this kit?
- The instruments and equipment required for the use of this kit are listed below;
- Deionized water (or distilled water)
- Test tubes for preparation of standard solution series
- Glassware for dilution of Wash Solution (10x) (a graduated cylinder, a bottle)
- Pipettes (disposable tip type). One should be able to deliver 50 μL precisely, and another for 100 – 1,000 μL
- Syringe-type repeating dispenser like Eppendorf multipette plus which can dispense 100 μL
- Paper towel to remove washing buffer remaining in wells
- A vortex-type mixer
- A shaker for 96 well-plate (600 – 1,200 rpm)
- An automatic washer for 96 well-plate (if available), or a wash bottle with a jet nozzle
- A 96 well-plate reader (450 ± 10 nm, 620 nm :600 - 650 nm)
- Software for data analysis
- How should I use the human IL-7 standard?
- The amount of purified water to be added varies by lot. Please reconstitute the human IL-7 standard with the amount of purified water specified in the “Reconstitution of standard” section on this product page to prepare the original standard solution (2,360 pg/mL). Then, dilute the solution using the kit-provided buffer that has been equilibrated to room temperature.
- What are the storage conditions for each reagent?
- [Buffer & TMB Solution]
Use only volume you need for your assay. Remaining reagents should be stored at 2-10℃ closing the cap tightly. Once opened, we recommend using as soon as possible to avoid influence by environmental condition.
[Stop Solution]
Close the cap tightly and store at 2-10℃.
[Human IL-7 Standard & Biotin-conjugated Antibody]
The reconstituted standard solution (original standard solution) should be used within 2 weeks if stored in a refrigerator. Dispose the remaining diluted standard solutions after use.
[Peroxidase-conjugated Streptavidin Solution]
Remaining working solution (already diluted) should be disposed. The rest of the undiluted solution (unused): close the cap tightly and store at 2-10℃. Once opened, we recommend using as soon as possible to avoid influence by environmental condition.
Prepare reagent solutions in appropriate volume for your assay.
Overview / Applications
Property
Manufacturer Information
Alias
- 637-50441(previous code)
AKH-IL7(previous code)
For research use or further manufacturing use only. Not for use in diagnostic procedures.
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