Anti CD133 Antibodies

CD133 (prominin-1) is a five-transmembrane glycoprotein with a molecular weight of approximately 120 kDa, encoded by the PROM1 gene in humans. In 1997, mouse prominin-1 was isolated from neuroepithelial precursor cells¹⁾, and its surface antigen AC133 (also known as CD133) was first reported as a surface marker for CD34-positive hematopoietic stem cells²⁾. Subsequently, CD133 was identified as one of the key markers of cancer stem cells (CSCs) derived from solid tumors³⁾. CD133 has been widely used for the isolation of normal stem cells and cancer stem cells from various tissues⁴⁾. Although little was known about the expression and functional regulation of CD133 in cancer, recent findings have identified CD133 as a target gene of p53, one of the most well-known tumor suppressors⁵⁾. Specifically, p53 directly binds to the CD133 promoter, suppressing its transcription, thereby inhibiting cancer cell survival and tumor formation⁵⁾.

Anti CD133, Monoclonal Antibody (CMab-43) is a mouse monoclonal antibody against human CD133⁶⁾.
It is suitable for use in immunohistochemistry, flow cytometry, and Western blotting.

Antibody Information

Application Data

• Sample: Colon cancer tissue
• Antibody concentration: 1 μg/mL

CMab-43 can be used to detect CD133-expressing cells when the human glioblastoma cell line LN229 or the Chinese hamster ovary cell subclone CHO-K1 is transfected with the CD133 gene.

Antibody concentration:

1 μg/mL

Secondary Antibody:

Alexa Fluor^® 488 fluorescently labeled anti-mouse IgG antibody (Alexa Fluor^® is a registered trademark of Invitrogen Inc.)

Note: The data for immunohistochemistry, flow cytometry and Western blotting are reproduced from reference 6) under CCBY 4.0.

Antibody concentration:

1 μg/mL

Secondary Antibody:

Peroxidase-conjugated anti-mouse IgG antibody

1. Weigmann, A. et al.: PNAS, 94 (23), 12425 (1997).
   Prominin, a novel microvilli-specific polytopic membrane protein of the apical surface of epithelial cells, is targeted to plasmalemmal protrusions of non-epithelial cells
2. Yin, A. H. et al.: Blood., 90 (12), 5002 (1997).
   AC133, a Novel Marker for Human Hematopoietic Stem and Progenitor Cells
3. Pleskač, P. et al.: Cell Mol. Biol. Lett., 29, 41 (2024) .
   Emerging roles of prominin-1 (CD133) in the dynamics of plasma membrane architecture and cell signaling pathways in health and disease
4. Irollo, E. and Pirozzi, G.: Am. J. Transl. Res., 5 (6), 563 (2013).
   CD133: to be or not to be, is this the real question?
5. Park, E. K. et al.: Cell death & disease, 6 (11), e1964 (2015).
   Transcriptional repression of cancer stem cell marker CD133 by tumor suppressor p53
6. Itai, S. et al.: Monoclon. Antib. Immunodiagn. Immunother., 36 (5), 231 (2017).
   Establishment of CMab-43, a Sensitive and Specific Anti-CD133 Monoclonal Antibody, for Immunohistochemistry