Veterinary Drug Mixture Standard Solutions by Drug Classification
Drugs used in animals are called veterinary drugs. Veterinary drugs are used for various animals such as pet animals, industrial animals, fish and poultry. Veterinary drugs are used to prevent and treatment of animal diseases, but in industrial animals including livestock, the drug remains in foods (meat, eggs, milk, seafood, etc.) which derived from dosed animals. Therefore, residual standard values have been set so that residual veterinary drugs do not damage human health. FUJIFILM Wako provides Veterinary Drug Mixture Standard, Veterinary Drug Mixture Standard Solutions by drug classification and PL series.
Veterinary drug mixture standard Solutions by drug classification are mixed with veterinary drugs by drug classification (quinolone drug, sulfa drug, macrolide, hormone drug).
Quinolone
LC/MS Chromatogram
| Peak No. | Constituent | Monitoring Ions (m/z) |
Mode | Peak No. | Constituent | Monitoring Ions (m/z) |
Mode |
|---|---|---|---|---|---|---|---|
| 1 | Marbofloxacin | 363 | + | 8 | Sarafloxacin* | 386 | + |
| 2 | Norfloxacin | 320 | + | 9 | Difloxacin | 400 | + |
| 3 | Ofloxacin | 362 | + | 10 | Oxolinic Acid | 262 | + |
| 4 | Ciprofloxacin* | 332 | + | 11 | Nalidixic Acid | 233 | + |
| 5 | Danofloxacin * | 358 | + | 12 | Flumequine | 262 | + |
| 6 | Enrofloxacin | 360 | + | 13 | Piromidic Acid | 289 | + |
| 7 | Orbifloxacin | 396 | + |
*The preparation concentration is converted to 20 µg/mL as free base.
Analysis conditions
[ Instrument ]
Shimadzu LC/MS-2020
[HPLC]
Column： Wakopak® Wakosil-Ⅱ 3C18HG 2.0 × 150 mm
Column temperature： 40 ℃
Eluent： A) 0.1 % HCOOH in H2O
B) CH3CN
Gradient：
| Time (min) | B conc. (%) |
|---|---|
| 0-10 | 1-10 |
| 10-35 | 10-100 |
| 35-40 | 100 |
Flow rate： 0.2 mL/min
[MS]
Ionization： ESI
Mode： TIC
Sulfonamide + Antifolate
LC/MS Chromatogram
| Peak No. | Constituent | Monitoring Ions (m/z) |
Mode | Peak No. | Constituent | Monitoring Ions (m/z) |
Mode | ||
|---|---|---|---|---|---|---|---|---|---|
| Precursor | Product | Precursor | Product | ||||||
| 1 | Sulfanilamide | 173 | 156 | + | 15 | Pyrimethamine | 249 | 233 | + |
| 2 | Sulfacetamide | 215 | 156 | + | 16 | Sulfamonomethoxine | 281 | 92 | + |
| 3 | Diaverdine | 261 | 245 | + | 17 | Sulfachlorpyridazine* | 285 | 156 | + |
| 4 | Sulfisomidine | 279 | 124 | + | 18 | Sulfamethoxazole | 254 | 92 | + |
| 5 | Sulfadiazine | 251 | 156 | + | 19 | Sulfadoxine | 311 | 156 | + |
| 6 | Trimethoprim | 291 | 230 | + | 20 | Sulfatroxazole | 268 | 92 | + |
| 7 | Ormetoprim | 275 | 259 | + | 21 | Sulfaethoxypyridazine | 295 | 156 | + |
| 8 | Sulfathiazole | 256 | 156 | + | 22 | Sulfisoxazole | 268 | 92 | + |
| 9 | Sulfapyridine | 250 | 156 | + | 23 | Sulfabenzamide | 277 | 156 | + |
| 10 | Sulfamerazine | 265 | 92 | + | 24 | Sulfadimethoxine | 311 | 156 | + |
| 11 | Sulfisozole* | 240 | 156 | + | 25 | Sulfaquinoxaline* | 301 | 156 | + |
| 12 | Sulfametoxydiazine | 281 | 92 | + | 26 | Sulfabromomethazine | 359 | 92 | + |
| 13 | Sulfadimidine | 279 | 92 | + | 27 | Sulfanitran | 336 | 156 | − |
| 14 | Sulfamethoxypyridazine | 281 | 156 | + | |||||
*The preparation concentration is converted to 20 µg/mL as free base.
Analysis conditions
[ Instrument ]
Shimadzu LC/MS-8040
[HPLC]
Column： Wakopak® Wakosil-Ⅱ 3C18HG 2.0 × 150 mm
Column temperature： 40 ℃
Eluent： A) 0.01 % HCOOH in H2O
B) CH3CN
Gradient：
| Time (min) | B conc. (%) |
|---|---|
| 0-25 | 2-45 |
| 25-35 | 45 |
Flow rate： 0.2 mL/min
[MS]
Ionization： ESI
Mode：MRM
Macrolide
LC/MS Chromatogram
| Peak No. | Constituent | Monitoring Ions (m/z) |
Mode | Peak No. | Constituent | Monitoring Ions (m/z) |
Mode |
|---|---|---|---|---|---|---|---|
| 1 | Neospiramycin I | 350 | + | 5 | Tylosin | 916 | + |
| 2 | Spiramycin I | 422 | + | 6 | Tiamulin* | 494 | + |
| 3 | Tilmicosin (mixture of isomers) | 870 | + | 7 | Leucomycin A5 | 772 | + |
| 4 | Erythromycin A | 734 | + |
*The preparation concentration is converted to 20 µg/mL as free base.
Analysis conditions
[ Instrument ]
Shimadzu LC-20AD
[HPLC]
Column： Wakopak® Ultra C18-3 2.0 × 100 mm
Column temperature： 40 ℃
Eluent： A) 0.5 % HCOOH in H2O
B) 0.5 % HCOOH in CH3CN
Gradient：
| Time (min) | B conc. (%) |
|---|---|
| 0-15 | 15-50 |
| 15-20 | 50 |
Flow rate： 0.2 mL/min
[MS]
Ionization： ESI
Mode： TIC
Hormone
LC/MS Chromatogram
| Peak No. | Constituent | Monitoring Ions (m/z) |
Mode | Peak No. | Constituent | Monitoring Ions (m/z) |
Mode |
|---|---|---|---|---|---|---|---|
| 1 | Prednisolone | 361 | + | 4 | α-Trenbolone | 271 | + |
| 2 | Methylprednisolone | 375 | + | 5 | Zeranol | 323 | + |
| 3 | β-Trenbolone | 271 | + | 6 | Clostebol | 323 | + |
Analysis conditions
[ Instrument ]
Shimadzu LC/MS-2020
[HPLC]
Column： Wakopak® Wakosil-Ⅱ 3C18HG 2.0 × 150 mm
Column temperature： 40 ℃
Eluent： A) 0.1 % HCOOH in H2O
B) CH3CN
Gradient：
| Time (min) | B conc. (%) |
|---|---|
| 0-20 | 30-70 |
| 20-30 | 70 |
Flow rate： 0.2 mL/min
[MS]
Ionization： ESI
Mode： TIC
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Mixed Standard Solutions
Standard Solutions
For research use or further manufacturing use only. Not for use in diagnostic procedures.
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