DNA Extractor® SP kit

for Genetic Research

Manufacturer :: FUJIFILM Wako Pure Chemical Corporation

Storage Condition :: Keep at 2-10 degrees C.

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	Distributor 296-60501 Barcode No 4987481439870	50Tests	List Price JPY 28,400	In stock in Japan	Certificate of Analysis

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Outline	This product is for research use only. Do not administer it to human.DNA extraction from serum and plasma DNA Extractor SP Kit efficiently extracts DNA fragments contained in serum and plasma. Based on the Sodium Iodide (NaI) method¹, this kit enables the whole procedures done in a single micro-centrifuge tube without using hazardous phenol/chloroform.Recently, tumor-specific genes have been amplified and detected in the serum and plasma of patients with various diseases such as lung, breast and colon cancer. Many of these reference articles are currently being published^2,3,4.The kit is a powerful pretreatment reagent for the detection and analysis of target DNA because of its high quality and yield. Features High DNA yield (about 100%) from small amount of serum and plasma The DNA extraction procedure is based on a simple centrifugal separation using Sodium Iodide and alcohol, that results in less loss of DNA. Safe operation No phenol or chloroform required. Minimum contamination: The entire extraction of DNA can be done in a single centrifuge tube Complete removal of lipid derived from blood Less variability in extraction from sample to sample Kit Contents (50 reactions) Enzyme reaction solution 1 x 10 mL Protein digestion solution 1 x 250 uL (uL: micro-L) Sodium iodide solution 1 x 15 mL Alcohol solution 1 x 30 mL Washing Solution (A) 1 x 50 mL Washing Solution (B) 1 x 50 mL References Ishizawa, M., Kobayashi, Y., Miyamura, T. and Matsuura, S.: Nucleic Acids Res., 22, 1774 (1994). Sozzi, G., Musso, K., Ratcliffe, C., Goldstraw, P., Pierotti, M. A. and Pastorino, U.: Clin. Cancer Res., 5, 2689 (1999) Silva, J. M., Dominguez, G., Garcia, J. M., Gonzalez, R., Villanueva M. J., Navarro, F., Provencio, M., San, Martin, S., Espana, P. and Bonilla, F.: Cancer Res., 59, 3251 (1999) Shao, Z. M., Wu, J., Shen Z. Z. and Nguyen, M.: Clin. Cancer Res., 7, 2222 (2001)
Features	1.High DNA yield from small amount of serum and plasmaThe DNA extraction procedure is based on a simple centrifugal separation using Sodium Iodide and alcohol, that results in less loss of DNA than in solid-phase2. Safety of operation - Hazardous phenol and chloroform are not employed as reagents3. Minimizing the cross and extraneous contaminationThe entire extraction of DNA is done in a single centrifuge tube.4. Complete removal of lipids derived from blood using a specialized alcohol solution.5. Extraction of high quality DNA without PCR inhibition
Instructions	*[100 uL (200 uL)^1) of serum or plasma^2)]* + 200 uL (300 uL) of Enzyme Reaction Solution and mix briefly + 5 uL (8-10 uL)^3) of Protein Digestion Solution and vortex Incubate at 56 degrees for 10 min. (at 56 degrees for 30 min.) + 300 uL (400 uL) of Sodium Iodide Solution and mix briefly + 600 uL (900 uL) of Alcohol Solution and vortex Incubate at R.T. for 10 min. Centrifuge at 12~20K x g at R.T. for 10 min.[Pellet]^4⁾ + 1 mL (1.5 mL)^5) of Washing Solution (A) to the pellet and vortex^6) Centrifuge at 12~20 K x g at R.T. for 5 min.[Pellet]^4) + 1 mL (1.5 mL) of Washing Solution (B) to the pellet and vortex^6) Centrifuge at 12~20 K x g at R.T. for 5 min.[Pellet]^4) Dry the pellet well for about 5 min. at 65 degrees C + Adequate volume (10~20 uL) of TE (pH 8.0) or D.W. (10~20 uL) and vortex^6) Dissolve the pellet completely with vortex mixer and incubate at 65 degrees C for 3~5 min.^7)[DNA Solution]: Store at 20 degrees C. Note:uL: micro litter1) Grouping symbol means the protocol from 200 uL of sample.2) Samples of serum and plasma should be placed on ice for the avoidance of activation of the endogenous DNase. We recommend Enzyme Reaction Solution to be added as soon as possible.3) Protein Digestion Solution should be placed on ice, during operation.4) Decant and remove the supernatant as much as possible from the tube that is inverted and placed on paper towel.5) It is possible to store the solution at - 20 degrees for long term.6) Mix vigorously until the pellet is removed from wall of tube.7) Dissolve the pellet completely with vortex mixer and incubation.

Outline

This product is for research use only. Do not administer it to human.DNA extraction from serum and plasma

DNA Extractor SP Kit efficiently extracts DNA fragments contained in serum and plasma. Based on the Sodium Iodide (NaI) method¹, this kit enables the whole procedures done in a single micro-centrifuge tube without using hazardous phenol/chloroform.Recently, tumor-specific genes have been amplified and detected in the serum and plasma of patients with various diseases such as lung, breast and colon cancer. Many of these reference articles are currently being published^2,3,4.The kit is a powerful pretreatment reagent for the detection and analysis of target DNA because of its high quality and yield.

Features

High DNA yield (about 100%) from small amount of serum and plasma
Safe operation No phenol or chloroform required.
Minimum contamination: The entire extraction of DNA can be done in a single centrifuge tube
Complete removal of lipid derived from blood
Less variability in extraction from sample to sample

Kit Contents (50 reactions)

Enzyme reaction solution 1 x 10 mL
Protein digestion solution 1 x 250 uL (uL: micro-L)
Sodium iodide solution 1 x 15 mL
Alcohol solution 1 x 30 mL
Washing Solution (A) 1 x 50 mL
Washing Solution (B) 1 x 50 mL

References

Ishizawa, M., Kobayashi, Y., Miyamura, T. and Matsuura, S.: Nucleic Acids Res., 22, 1774 (1994).
Sozzi, G., Musso, K., Ratcliffe, C., Goldstraw, P., Pierotti, M. A. and Pastorino, U.: Clin. Cancer Res., 5, 2689 (1999)
Silva, J. M., Dominguez, G., Garcia, J. M., Gonzalez, R., Villanueva M. J., Navarro, F., Provencio, M., San, Martin, S., Espana, P. and Bonilla, F.: Cancer Res., 59, 3251 (1999)
Shao, Z. M., Wu, J., Shen Z. Z. and Nguyen, M.: Clin. Cancer Res., 7, 2222 (2001)

Features

1.High DNA yield from small amount of serum and plasmaThe DNA extraction procedure is based on a simple centrifugal separation using Sodium Iodide and alcohol, that results in less loss of DNA than in solid-phase2. Safety of operation - Hazardous phenol and chloroform are not employed as reagents3. Minimizing the cross and extraneous contaminationThe entire extraction of DNA is done in a single centrifuge tube.4. Complete removal of lipids derived from blood using a specialized alcohol solution.5. Extraction of high quality DNA without PCR inhibition

Instructions

[100 uL (200 uL)^*1) of serum or plasma^*2)] + 200 uL (300 uL) of Enzyme Reaction Solution and mix briefly + 5 uL (8-10 uL)^*3) of Protein Digestion Solution and vortex Incubate at 56 degrees for 10 min. (at 56 degrees for 30 min.) + 300 uL (400 uL) of Sodium Iodide Solution and mix briefly + 600 uL (900 uL) of Alcohol Solution and vortex Incubate at R.T. for 10 min. Centrifuge at 12~20K x g at R.T. for 10 min.[Pellet]^*4⁾ + 1 mL (1.5 mL)^*5) of Washing Solution (A) to the pellet and vortex^*6) Centrifuge at 12~20 K x g at R.T. for 5 min.[Pellet]^*4) + 1 mL (1.5 mL) of Washing Solution (B) to the pellet and vortex^*6) Centrifuge at 12~20 K x g at R.T. for 5 min.[Pellet]^*4) Dry the pellet well for about 5 min. at 65 degrees C + Adequate volume (10~20 uL) of TE (pH 8.0) or D.W. (10~20 uL) and vortex^*6) Dissolve the pellet completely with vortex mixer and incubate at 65 degrees C for 3~5 min.^*7)[DNA Solution]: Store at 20 degrees C.

Note:uL: micro litter*1) Grouping symbol means the protocol from 200 uL of sample.*2) Samples of serum and plasma should be placed on ice for the avoidance of activation of the endogenous DNase. We recommend Enzyme Reaction Solution to be added as soon as possible.*3) Protein Digestion Solution should be placed on ice, during operation.*4) Decant and remove the supernatant as much as possible from the tube that is inverted and placed on paper towel.*5) It is possible to store the solution at - 20 degrees for long term.*6) Mix vigorously until the pellet is removed from wall of tube.*7) Dissolve the pellet completely with vortex mixer and incubation.

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