DNA ExtractorR FM Kit

Identification of individual by DNA analysis is powerful method in forensic medicine. However, samples for forensic DNA analyses obtained in the scene are often insoluble materials such as body hairs, nails, saliva stains and blood stains, which usually contain quite limited amounts of DNA. Therefore, it is sometimes difficult to examine DNA due to complicated hard isolation procedures or its insufficient recovery. This kit provides a rapid procedure for isolation of DNA from such the rigid materials, overcoming difficulties described above by combining rapid solubilization of DNA from the rigid materials with our original NaI DNA extraction procedure without use of harmful organic solvents such as phenol and chloroform.

[Assay Materials]Body hair (2 pieces of 1 cm long or the less) nail (0.5 mg or the less), saliva stain and blood stain

[Features]

1. Solubilizes a variety of tough hairs as body hair completely within several ten minutes.
2. Useless of harmful organic solvents such as phenol and chloroform
3. Avoids loss of DNA form limited amounts of sample DNA unlike solid phase DNA extraction by non-specific absorption of DNA to solid support.
4. Attains DNA purification with nearly 100% recovery by using NaI-DNA Extraction.
5. Ready to use DNA preparation obtained by the kit in DNA amplification as the polymerase chain reaction.
6. Provides a set of essential reagents for the procedure with the kit.

1. Lysis Solution 9.5 mL
2. Enzyme-activated Reagent (EAR) 80 mg
3. Reconstitution Solution for EAR 0.5 mL
4. Protease 10 mg
5. Sodium Iodide Solution 12.5 mL
6. Washing Solution (A) 50 mL
7. Washing Solution (B) 50 mL