Chromogranin A ELISA Kits
Chromogranin A (CgA) is an acidic glycoprotein that occurs in the adrenal medulla and sympathetic nerve endings. Characterized by coexistence and co-release with catecholamine, CgA is used as an activity index for the sympathetic-adrenal-medullary (SAM) axis, a stress-response pathway. In particular, saliva CgA is expected to be an excellent biomarker for noninvasive measurement of mental stress. Fujifilm Wako supply an ELISA kit for assaying CgA in human saliva and plasma.
What is chromogranin A?
Chromogranin A (CgA) is an acidic glycoprotein that coexists and is co-released with catecholamine in the adrenal medulla and sympathetic nerve endings. CgA is thought to reflect secretion of catecholamine and is used as an activity index for the sympathetic-adrenal-medullary (SAM) axis, a stress-response pathway. It is also used as a tumor marker for patients with pheochromocytomas or neuroendocrine tumors, who have high levels of CgA in the blood.
CgA is also present in saliva, is synthesized in the submandibular gland, and is released into saliva in response to autonomic stimulation. Previous studies have reported that saliva CgA levels increase in response to stress1) 2), and thus, it is particularly useful as an excellent noninvasive biomarker for mental stress.
Human Chromogranin A ELISA Kit Wako
Human Chromogranin A ELISA Kit Wako is an ELISA kit for assaying CgA in human saliva and plasma.
Kit Performance
The range of the calibration curve | 0.14 - 33.33 pmol/mL |
---|---|
Assay target | Chromogranin A |
Sample | Human Saliva/Plasma |
The sample amount required | 50 µL (n=2) |
Assay time | O/N+2.5 hours |
Detection method | Colorimetry |
Within assay variation | CV< 14% |
Between assay variation | CV< 16% |
Example of Calibration Curve
![03101_img01.png](../../us/category/images/03101_img01.png)
Performance Data
Sample | Amount spiked (pmol/mL) |
Measured value (pmol/mL) |
Recovery rate (%) |
---|---|---|---|
Plasma | 0 | 0.54 | - |
0.25 | 0.86 | 108.86 | |
1 | 1.93 | 125.32 | |
4 | 6.58 | 144.93 | |
Saliva | 0 | 0.47 | - |
0.25 | 0.81 | 112.50 | |
1 | 1.40 | 95.24 | |
4 | 3.66 | 81.88 |
References
- 1) Nakane, H. et al.: Biomed. Res., 19(6), 401(1998).
- Salivary chromogranin A as an index of psychosomatic stress response
- 2) Takatsuji, K. et al.: Biomed. Res., 29(4), 221(2008).
- The effects of examination stress on salivary cortisol, immunoglobulin A, and chromogranin A in nursing students
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