s-IgA ELISA Kits

Secretory IgA (s-IgA) is a type of immunoglobulin that mainly occurs in exocrine secretion and plays an important role in protection from infection on the mucosa of the respiratory and gastrointestinal tracts. The level of s-IgA in human saliva is reportedly increased by acute stress and decreased by chronic stress, giving it utility as a stress biomarker. Fujifilm Wako supply an ELISA kit for measuring s-IgA levels in human saliva.

What is secretory IgA (s-IgA)?

Immunoglobulin A (IgA) is a type of immunoglobulin that provides protection from infection. It is mainly distributed in serum and exocrine gland secretions. After IgG, it is the second most abundant immunoglobulin in serum and the most abundant immunoglobulin in exocrine secretions. Human IgA, which has two subclasses, IgA1 and IgA2, occurs mainly as a monomer in serum and as a dimer in secretions1).

Secretory IgA (s-IgA) is an IgA to which a secretory component (SC) is bound, basically occurring as a dimer but also as tetramer, etc. IgA in secretions occurs principally as s-IgA, which plays an important role in protection from infection on the mucosa of the respiratory and gastrointestinal tracts.

s-IgA is also found in human saliva and produced in plasma cells in the salivary glands. The level of s-IgA in human saliva is reportedly increased by acute stress2) and decreased by chronic stress3) 4), giving it utility as a stress biomarker.

Human s-IgA ELISA Kit Wako

Human s-IgA ELISA Kit Wako is an ELISA kit for assaying s-IgA in human saliva.

  • Kit Performance

    The range of
    the calibration curve
    0.082 - 20 µg/mL
    Assay target Secretory IgA (s-IgA)
    Sample Human Saliva
    The sample
    amount required
    5 µL
    Assay time 2.5 hours
    Detection method Colorimetry
    Within assay variation CV< 6%
    Between assay variation CV< 12%
  • Example of Calibration Curve

    03100_img01.png

Performance Data

  • Spiked recovery test

    Sample Amount spiked
    (µg/mL)
    Measured value
    (µg/mL)
    Recovery rate
    (%)
    Saliva1 0 64.8
    40 108.8 103.8
    160 220.8 98.2
    400 404.8 87.1
    Saliva 2 0 46.4
    40 96.0 111.1
    160 196.0 95.0
    400 432.0 96.8
    Saliva 3 0 120.8
    40 189.6 117.9
    160 274.4 97.7
    400 476.0 91.4
    Saliva 4 0 112.0
    40 157.6 103.7
    160 244.8 90.0
    400 441.6 86.3
    Saliva 5 0 105.6
    40 152.0 104.4
    160 250.4 94.3
    400 600.8 118.8

    Cross reactivity with various types of immunoglobulins

    Immunoglobulins Cross-reactivity (%)
    Serum IgG(Human) <0.1
    Serum IgA(Human) 0.3
    Serum IgM(Human) 0.0
    Serum IgE(Human) 0.0

References

  1. de Sousa-Pereira, P. and Woof, J. M.: Antibodies, 8(4), 57(2019).
    IgA: Structure, Function, and Developability
  2. Viena, T. D. et al.: Biol. Psychol., 91(2), 307(2012).
    Differential effects of mild chronic stress on cortisol and S-IgA responses to an acute stressor
  3. Deinzer, R. et al.: Int. J. Psychophysiol., 37(3), 219(2000).
    Prolonged reduction of salivary immunoglobulin A (sIgA) after a major academic exam
  4. Phillips, A. C. et al.: Brain, Behav. Immun., 20(2), 191(2006).
    Stressful life events are associated with low secretion rates of immunoglobulin A in saliva in the middle aged and elderly

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