Reagents, kits, and related products for SARS-CoV-2 testing
The novel Coronavirus SARS-CoV-2; Severe Acute Respiratory Syndrome Coronavirus 2 is the causative virus of the Coronavirus Disease 2019 (COVID-19). The World Health Organization (WHO) declared the COVID-19 outbreak as a global pandemic on March 11, 20201-2.
SARS-CoV-2 is an enveloped and positive single-stranded RNA virus3. The process of SARS-CoV-2 entering into the host cells begins through the binding of the viral spike (S) protein to the receptors, the Angiotensin-converting enzyme 2 (ACE-2) and dipeptidyl peptidase IV (DPP4), on Ciliated Bronchial Epithelial Cells or Type II Pneumocytes, and then are followed to the endocytosis pathway4.
Currently, the development of DNA vaccines targeting spike (S) proteins, recombinant protein vaccines, viral vector vaccines, live attenuated vaccines targeting the whole virion, and inactivated vaccines are underway5.
Multiple clinical trials are also in progress in the therapeutic drug development6. Therapeutic agents can be divided into two types depending on the target. One is that it acts directly on the virus and inhibits virus growth or virus invasion into human cells. The other is to relieve symptoms by increasing the immune response to the virus or inhibiting the inflammatory process.
FUJIFILM Wako introduces research reagents and test kits to support vaccine and therapeutic drug development and manufacturing for COVID-19.
- SARS-CoV-2 detection from samples
SARS-CoV-2 detection from samples
Product Code Manufacturer Product Name Product Introduction 283-33209 FUJIFILM Wako SARS-CoV-2 Real-Time RT-PCR Detection Kit(N gene) SARS-CoV-2 Real-Time RT-PCR Detection Kits a reagent system, based on an one-step real-time PCR method, for the qualitative detection of SARS-CoV-2-derived RNA. The highly active enzyme applied in the kit allows high-speed real-time PCR and it plays dual roles as reverse transcriptase and DNA polymerase.
-RT-PCR only for 45 minutes
-Inhibiting non-specific amplification by hot start PCR
-Including positive controls
281-34501 FUJIFILM Wako SARS-CoV-2 Lysis Buffer Ver.2 SARS-CoV-2 Lysis Buffer ver.2 is SARS-CoV-2-derived RNA extraction buffer from saliva or nasopharyngeal swab for real-time RT-PCR. SARS-CoV-2 can be lysed quickly and efficiently by this product which mainly contains a protease and surfactant. A simple and easy protocol with it provides reliable results, shortening time for assays and risk reduction from being infected with SARS-CoV-2. Using SARS-CoV-2 Real-Time RT-PCR Detection Kit(N gene)(Code No. 283-33209) with this product is recommended.
- Extraction of viral RNA
Extraction of viral RNA
ISOSPIN Viral RNA
Product Code Manufacturer Product Name Product Introduction 310-08931 Nippon Gene Co., Ltd. ISOSPIN Viral RNA ISOSPIN Viral RNA kit is designed for the rapid preparation of viral RNA from fluid biological samples, such as nasopharyngeal and oropharyngeal swab washes, serum, saliva, and sputum.
With the ISOSPIN Viral RNA method, RNA viruses are lysed quickly by NIRV Extraction Buffer and Proteinase K. Ethanol is added to the lysate and the lysate and ethanol create appropriate conditions for binding of RNA to the silica-based membrane of the Spin Column. Impurities are removed by washing with two kinds of Wash Buffers. The RNA is eluted in RNase-free water.
The Spin Column of the ISOSPIN Viral RNA kit enables a high loading capacity. ISOSPIN Viral RNA kit allows purification of pure RNA in 30 minutes without phenol/chloroform extraction or alcohol precipitation.
- Hygiene products
Product Code Manufacturer Product Name Product Information 053-09375 FUJIFILM Wako 80vol% Ethanol Ethanol for sterilization with ethanol concentration (77-82 vol%). Ideal for disinfecting saliva test and research environments. 181-03381 FUJIFILM Wako RNase Knockout RNase Knockout is a reagent which inactivates RNase. Use it when you are concerned about contamination of RNase such as RNA treatment experiments. The method of usage is simple. It can be inactivated by spraying it on laboratory instruments and laboratory bases and wiping it off.
Product Code Manufacturer Product Name Product Information 294-67501 FUJIFILM Wako Open Essence A card-type measurement kit that allows the identification of odor easily. Standard olfactory tests and venous (hematological) olfactory tests are used to measure olfaction, but it is desirable to develop a simpler measurement method because the odor spreads and it takes time to measure. This product does not diffuse odors and can be easily measured.
- Coronavirus disease (COVID-19) pandemic. (2020). Accessed: March 20, 2020:
- Coronavirus disease 2019 (COVID-19): situation summary. (2020). Accessed: March 20, 2020:
- Kahn J, McIntosh K: History and recent advances in coronavirus discovery. Pediatr Infect Dis J. 2005, 24:S223-S227.
- Cui J, Li F, Shi Z: Origin and evolution of pathogenic coronaviruses. Nat Rev Microbiol. 2019, 17:181-192.
- Amanat F, Krammer F. SARS-CoV-2 Vaccines: Status Report. Immunity. 2020, 52(4):583-589.
- Tu, Y.-F et al.:A Review of SARS-CoV-2 and the Ongoing Clinical Trials. Int. J. Mol. Sci. 2020, 21, 2657.