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SARS-CoV-2 Real-Time RT-PCR Detection Kit(N gene)

for Genetic Research
Manufacturer :
FUJIFILM Wako Pure Chemical Corporation
Storage Condition :
Keep at -20 degrees C.
GHS :
  • Structural Formula
  • Label
  • Packing
SDS
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Product Number
Package Size
Price
Inventory
Distributor
283-33209
Barcode No
4548995082077
200reactions(For 100samples)
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Document

SDS
Package Insert
Spectral Data
Certificate of Analysis
Calibration Certificate

Kit component

Kit components (for 200 reactions)

5×Reaction Buffer 800 μL
2mmol/L dNTPs 800 μL
50mmol/L Manganese(II) Acetate 200 μL
Hot Start Reverse Transcription DNA Polymerase 50 μL
Distilled Water, Nuclease-free 590 μL
Fw and Rv Primers 1 100 μL
TaqMan Probe 1 40 μL
Fw and Rv Primers 2 100 μL
TaqMan Probe 2 40 μL
Positive Control RNA, N gene 1,000 μL
*A ROX for passive reference dye is not supplied in this kit. Please prepare it as needed. TaqMan is registered trademark by Roche Diagnostics K.K. -

Materials required but not provided

Real-Time PCR instrument -
Microcentrifuge -
Micro pipette and nuclease-free pipette tips -
Nuclease-free 1.5mL tubes(e.g. Micro tube 1.5ml DNA LowBind, Sarstedt) -
Real-time PCR plates and plate films or real-time PCR tubes and caps. -
TaqMan® is registered trademark by Roche Diagnostics K.K. -

Overview

Product description

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SARS-CoV-2 Real-Time RT-PCR Detection Kits a reagent system, based on an one-step real-time PCR method, for the qualitative detection of SARS-CoV-2-derived RNA. The highly active enzyme applied in the kit allows high-speed real-time PCR and it plays dual roles as reverse transcriptase and DNA polymerase.

The primer/probe No.1 and 2 are designed to hybridize to N(Nucleocapsid) gene and labeled with the FAM.

Key Features

  • RT-PCR only for 45 minutes
  • Inhibiting non-specific amplification by hot start PCR
  • Including positive controls

Two targets at N gene

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Protocol flow

    1. Prepare extracted RNA from SARS-CoV-2.
    2. Dilute Positive Control RNA with water.
    3. Prepare PCR master mix.
    4. Apply PCR master mix and RNA into real-time PCR plate or tube.
    5. Run a real-time PCR instrument.

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    Probe Reporter
    TaqMan® Probe 1 FAM
    TaqMan® Probe 2 FAM
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Example of arrangement of applying samples in 96-well plate

1 2 3 4 5 6 7 8 9-12
A
B P S1 & No.1 S2 & No.1 S3 & No.1 S4 & No.1 S5 & No.1
C N S1 & No.2 S2 & No.2 S3 & No.2 S4 & No.2 S5 & No.2
D
E
F
G
H

S:Sample
No.1:Fw & Rv Primers 1, TaqMan® Probe 1
No.2:Fw & Rv Primers 2, TaqMan® Probe 2
P : Positive control
N : Negative control

Two wells are used for one sample

Example applications

Positive control RNA, N=2

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CFX96 Touch Real-Time PCR Detection System


Positive control RNA, N=2

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CFX96 Touch Real-Time PCR Detection System

Standard Curve

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Manufacturer Information

Alias

Product content may differ from the actual image due to minor specification changes etc.

If the revision of product standards and packaging standards has been made, there is a case where the actual product specifications and images are different.

877-714-1920

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