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SARS-CoV-2 Real-Time RT-PCR Detection Kit(N gene)

for Genetic Research
Manufacturer :
FUJIFILM Wako Pure Chemical Corporation
Storage Condition :
Keep at -20 degrees C.
GHS :
  • Structural Formula
  • Label
  • Packing

If the revision of product standards and packaging standards has been made, there is a case where the actual product specifications and images are different.

SDS

Basic Information

Comparison
Product Number
Package Size
Price
Inventory
Distributor
283-33209
Barcode No
4548995082077
200reactions(For 100samples)
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Document

SDS
Package Insert
Spectral Data
Certificate of Analysis
Calibration Certificate

Kit component

Kit components (for 200 reactions)

5×Reaction Buffer 800 μL
2mmol/L dNTPs 800 μL
50mmol/L Manganese(II) Acetate 200 μL
Hot Start Reverse Transcription DNA Polymerase 50 μL
Distilled Water, Nuclease-free 590 μL
Fw and Rv Primers 1 100 μL
TaqMan Probe 1 40 μL
Fw and Rv Primers 2 100 μL
TaqMan Probe 2 40 μL
Positive Control RNA, N gene 1,000 μL
*A ROX for passive reference dye is not supplied in this kit. Please prepare it as needed. TaqMan is registered trademark by Roche Diagnostics K.K. -

Materials required but not provided

Real-Time PCR instrument -
Microcentrifuge -
Micro pipette and nuclease-free pipette tips -
Nuclease-free 1.5mL tubes(e.g. Micro tube 1.5ml DNA LowBind, Sarstedt) -
Real-time PCR plates and plate films or real-time PCR tubes and caps. -
TaqMan® is registered trademark by Roche Diagnostics K.K. -

Overview

Product description

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SARS-CoV-2 Real-Time RT-PCR Detection Kits a reagent system, based on an one-step real-time PCR method, for the qualitative detection of SARS-CoV-2-derived RNA. The highly active enzyme applied in the kit allows high-speed real-time PCR and it plays dual roles as reverse transcriptase and DNA polymerase.

The primer/probe No.1 and 2 are designed to hybridize to N(Nucleocapsid) gene and labeled with the FAM.

Key Features

  • RT-PCR only for 45 minutes
  • Inhibiting non-specific amplification by hot start PCR
  • Including positive controls

Two targets at N gene

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Protocol flow

    1. Prepare extracted RNA from SARS-CoV-2.
    2. Dilute Positive Control RNA with water.
    3. Prepare PCR master mix.
    4. Apply PCR master mix and RNA into real-time PCR plate or tube.
    5. Run a real-time PCR instrument.

    04007859_img03.png


    Probe Reporter
    TaqMan® Probe 1 FAM
    TaqMan® Probe 2 FAM
  • 04007859_img04.png

Example of arrangement of applying samples in 96-well plate

1 2 3 4 5 6 7 8 9-12
A
B P S1 & No.1 S2 & No.1 S3 & No.1 S4 & No.1 S5 & No.1
C N S1 & No.2 S2 & No.2 S3 & No.2 S4 & No.2 S5 & No.2
D
E
F
G
H

S:Sample
No.1:Fw & Rv Primers 1, TaqMan® Probe 1
No.2:Fw & Rv Primers 2, TaqMan® Probe 2
P : Positive control
N : Negative control

Two wells are used for one sample

Example applications

Positive control RNA, N=2

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CFX96 Touch Real-Time PCR Detection System


Positive control RNA, N=2

04007859_img04R.png

CFX96 Touch Real-Time PCR Detection System

Standard Curve

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Manufacturer Information

Alias

Product content may differ from the actual image due to minor specification changes etc.

877-714-1920

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