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Detail Information
  1. Top
  2. Life Science
  3. Protein Research
  4. Protein Electrophoresis Reagents
  5. Gel Stain Reagents (Silver Staining)
  6. Silver Stain MS Kit

Silver Stain MS Kit

for Electrophoresis
Manufacturer :
FUJIFILM Wako Pure Chemical Corporation
Storage Condition :
Keep at 2-10 degrees C. (RT)
GHS :
Citations (11)
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SDS
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Product Number
Package Size
Price
Availability
Certificate of Analysis
Purchase
Distributor
299-58901
Barcode No
4987481416901
20Tests
List Price
209.00 USD

In stock in Japan
Distributor

※Check  availability  in the US with the distributor.

Document

SDS
Product Specification Sheet
Spectral Data
Certificate of Analysis
Calibration Certificate

Application

Overview / Applications

Outline This product is for research use only. Do not administer it to human.

This silver staining kit is produced for mass spectrometric sequencing of protein separated by polyacrylamide gel electrophoresis, based on the method described by Shevchenko, et al. By silver staining, protein is rarely modified chemically due to omitting treatment of glutaraldehyde and is detected at several nanograms level on the elecrophoretic gel.

[Features]

  • Detectable limit of protein: 1 ng
  • ideal for MS analysis because rarely modified protein is obtained due to omitting glutaraldehyde treatment.
Procedure[SDS-PAGE gel]
  1. Fixing Soln. 1
    2. Shake 20 minutes
  2. Fixing Soln. 2
    3. Shake 10 minutes and wash 10 minutes.
  3. Enhancing Soln.
    4. Shake 1 minute and wash 2 x 1 minute.
  4. Staining Soln.
    5. Shake 20 minutes and wash 2 x 1 minute.
  5. Developing Soln.
    6. Shake 3 ~ 10 minutes.
  6. Stopper
    7. Shake 1 minute and wash 3 x 1 minute.
[The excised band
  1. De-staining mixture
    2. Leave 15 minutes.
[Mass Spectrometry]

[Kit Contents]

  1. Enhancing Stock Soln. 1 bottle x 200 mL
  2. Staining Stock Soln. 1 bottle x 200 mL
  3. Developing Stock Soln. 1 bottle x 100 mL
  4. Developing Powder 1 bottle x 20 g
  5. Stopper 1 bottle x 200 mL
  6. De-staining Soln.A 1 bottle x 50 mL
  7. De-staining Soln.B 1 bottle x 50 mL
[References]
  1. Shevchenko,A., et al.:Anal.Chem. 68, 850 (1996)
  2. Gharahdaghi,F., et al.:Electrophoresis, 20, 601 (1999)

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Manufacturer Information

Alias

For research use or further manufacturing use only. Not for use in diagnostic procedures.

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