Cytotoxic Fluoro test Wako
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1000Tests
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Overview / Applications
| Outline | This product is for research use only. Do not administer it to human. This test provides a simple protocol and the necessary reagents to measure viability on cells treated with various cytotoxic substrates. It is based on a fluorometric assay of cell lysis and screening (FACLS). Proliferation and aging of cells can also be estimated by this procedure. The test utilizes properties of the fluorescent dye that differentiates between intact cells and damaged cells. The dye permeate only damaged or dead cell membranes and reacts with DNA in the cells. Measurement of the intensity of fluorescence emissions of the DNA-dye complex before and after addition of the cell lysis reagent allows determination of the number of both damaged cells and total cells in the sample. The procedure is applicable to adhesive cells as well as floating cells. The following are applicable fields: Cytotoxicity assays Apoptosis and necrosis (determination of ratio of dead cells and living cells) Cell proliferation and aging Contents: Fluorescence Solution 500 uL x 1 vial Lysis Solution 10 mL x 1 vial |
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| Kit Components | Kit Contents1. Fluorescence Reagent 500 ug x 1 vial2. Lysis Solution 10 mL x 1 vial |
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For research use or further manufacturing use only. Not for use in diagnostic procedures.
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