Silver Protein
- for Pathology Research
- Manufacturer :
- FUJIFILM Wako Pure Chemical Corporation
- Storage Condition :
- Keep at RT.
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- Structural Formula
- Label
- Packing
- SDS
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Comparison
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Product Number
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Price
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Availability
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Certificate of Analysis
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Purchase |
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3G
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In stock in Japan |
※Check availability in the US with the distributor.
Document
Stained sample
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Fig. 1 Cerebellum
Axon (black), Purkinje cell (reddish purple),
granulocyte (reddish purple). -
Fig 2 Cerebrum
Senile plaque (reddish purple) and amyloid core (red)
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Fig.3 Cerebrum
Neurofibrillary tangle (arrow, reddish purple - blackish brown) -
Fig. 4 Cerebrum (Pick disease)
Pick body (arrow, reddish brown)
Staining property
Axon: black
Purkinje cell and granular layer (cerebellum): reddish purple
Cerebellar cortex: light purple
Histologic features in neurodegenerative disease
Senile plaque: reddish purple (amyloid core: red)
Neurofibrillary tangle: neurofibrillary tangle (red - blackish purple)
Pick body (Pick disease): reddish brown
Examples of staining protocol
Overview of Bodian staining
Bodian staining protocol
1．1% Silver protein solution |
2．Copper particles |
| Weigh 0.5 g of silver protein into a Coplin jar, and add 50 mL of distilled water. Wait for dissolution (for about 1 hour).
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Add 4 g of copper particles.
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3．Place the slide to be tested in the Coplin jar. |
4．Allow to stand overnight in 40°C incubator (for about 12-18 hrs). |
| Approximately 6 slides per 50 mL are recommended.
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Caution should be exercised to avoid excessive black coloration due to an excessive silver reaction caused by an extended incubation.
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5．Preparation of reducing solution |
6．Reduction treatment |
| 【Composition】 Distilled water・・・100 mL Hydroquinone・・1 g Formalin・・・5 mL |
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7．Gold chloride treatment |
8．1% Oxalic acid treatment |
| After reduction treatment, wash with water for 3 minutes followed by the treatment below. The sample turns white first, followed gradually by brownish coloration.
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Reddish purple coloration is gradually seen.
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9．Fixing |
10．Dehydration, clearing, mounting |
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Precautions
- Standard procedures may be used for staining, but please avoid extended silver reaction.
- Because successful staining requires appropriate temperature and time, please use experimental conditions well adapted to your institution.
- Fixation performance is highest with acidic formalin, followed by neutral buffered formalin, and then by paraformaldehyde; an extended fixation time is not expected to affect fixation performance.
Overview / Applications
| Outline | This product is for research use only. Do not administer it to human.
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Property
| Appearance | Slightly brown - brown, crystalline powder - powder or mass |
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Manufacturer Information
Alias
For research use or further manufacturing use only. Not for use in diagnostic procedures.
Product content may differ from the actual image due to minor specification changes etc.
If the revision of product standards and packaging standards has been made, there is a case where the actual product specifications and images are different.