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SuperSep™ Phos-tag™ (50μmol/L), 12.5%, 17well, 100×100×6.6mm

for Electrophoresis
Manufacturer :
FUJIFILM Wako Pure Chemical Corporation
Storage Condition :
Keep at 2-10 degrees C.
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List Price
330.00 USD

In stock in Japan


Product Specification Sheet
Spectral Data
Certificate of Analysis
Calibration Certificate


  • Ready-to-use, saves time
  • Able to isolate phosphorylated proteins by the level of phosphorylation
  • Good separation with sharp bands


Albumin Dephosphorylation


[Electrophoresis Buffer] Tris-Glycine SDS Buffer
[Electrophoresis Samples]
 Lane1: Untreated Albumin
 Lane2: Dephosphorylated Albumin
[Electrophoresis cConditions] 20 mA, 70 mins
[Staining] Quick CBB staining
[Destaining] Deionized wWater

Albumin (Cat. No. 010-17071) was dephosphorylated using alkaline phosphatase (NIPPON GENE CO., LTD., Cat. No. 319-02661). Band shift confirmed dephosphorylation of albumin.

Time-dependent Dephosphorylation of β-casein


[Electrophoresis Buffer] Tris-Glycine SDS buffer
[Electrophoresis Samples]
 Lane1: β-casein (AP-treatment: 0 min)
 Lane2: β-casein (AP-treatment: 15 min)
 Lane3: β-casein (AP-treatment: 30 min)
 Lane4: β-casein (AP-treatment: 45 min)
 Lane5: β-casein (AP-treatment: 60 min)
[Electrophoresis Conditions] 35mA, 60min
[Staining] Quick CBB staining
[Destaining] Deionized Water

Dephosphorylation of β-casein was performed over different time periods. The results confirmed separation of phosphorylated and dephosphorylated β-casein.
In addition, the results demonstrated the level of dephosphorylation across different time points.

Time-dependent Change in the level of phosphorylation

Data was provided by courtesy of Dr. Atsushi Enomoto, Molecular Radiology / Sec. of Radiation Biology, Center for Disease Biology and Integrative Medicine, Faculty of Medicine, University of Tokyo

X-ray irradiation (5 Gy) was performed for human lung cancer cell line Lu99, and cells were collected across different time points. Cell extracts were prepared, and SDS-PAGE was performed in 13 wells using 50 μmol/L of 10% SuperSep™ Phos-tag™

Gentle agitation of the gel was performed in a transfer buffer containing 10 mmol/L EDTA, and proteins were transferred to PVDF membrane. The membrane was blocked with 2% Milk/TBS-T, and was reacted with primary antibodies (upper image: p53, lower image: cell-cycle proteins). Detection was performed by chemoluminecence.


Protein accumulation for p53 was at the highest level 4 hours after X-ray irradiation. The level of protein X phosphorylation changed over time after X-ray irradiation.

Electrophoresis of Control Protein


P10 %(left): SuperSep™Phos-tag (50 μmol/L), 10 %, 13Wells
P15 %(middle): SuperSep™Phos-tag (50 μmol/L), 15 %, 13Wells
A15 %(right): SuperSep™Ace, 15 %, 13Wells

Electrophoresis Conditions

30 mA/gel (constant), 60 mins


5 μg/lane of α-casein from bovine milk, dephosphorylated (Cat. No. 038-23221)

  • The product contains α-casein that hasve not been dephosphorylated. While conventional SDS-PAGE will only show a single main band, Phos-tag SDS-PAGE will show two main bands for α-casein and dephosphorylated α-casein.
Running Buffer


Quick CBB Plus (Cat. No. 174-00553)

Overview / Applications

Outline SuperSep Phos-tag are precast polyacrylamide gels containing Phos-tag for SDS-PAGE. Phos-tag is a functional molecule that can bind specifically to Phosphate groups.
This product can trap phosphorylated proteins during SDS-PAGE and decrease the mobility of phosphorylated proteins. It allows detection of certain phosphorylated and non-phosphorylated proteins as different bands.

This product is designed for XCell SureLock Mini-Cell, Life Technologies's electrophoresis tank.
XCell SureLock is a registered trademark of Life Technologies, Inc.

* This product is for oversea sales only. It is not available in Japan.


Concentration Acrylamide concetration: 12.5 %

Manufacturer Information


Product content may differ from the actual image due to minor specification changes etc.

If the revision of product standards and packaging standards has been made, there is a case where the actual product specifications and images are different.


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