Anti Segatella, Monoclonal Antibody (SC-S10C3-49K1)
- for Immunochemistry
- Manufacturer :
- FUJIFILM Wako Pure Chemical Corporation
- Storage Condition :
- Keep at -20 degrees C.
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50uL
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In stock in Japan |
※Check availability in the US with the distributor.
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Product Overview
Developed by Dr. Jun Kunisawa and Dr. Ken Yoshii at the National Institutes of Biomedical Innovation, Health and Nutrition, Fujifilm Wako now offers monoclonal antibody against Segatella copri1) (formerly Prevotella copri). It is suitable for use in ELISA, flow cytometry, immunoprecipitation, and Western blotting, allowing for rapid, simple, and cost-effective detection of gut bacteria.
Antibody Information
| Clonality | Monoclonal |
|---|---|
| Antigen | Heat-treated Segatella copri |
| Host | Mouse |
| Formulation | 1 x D-PBS, 0.05% Sodium Azide |
| Conjugate | Unlabeled |
| Cross-reactivity | Segatella copri (gdhA) |
| Application | ELISA (Direct/Sandwich) Flow Cytometry Immunoprecipitation Western Blotting |
Data
Quantification of Individual Gut Bacterial Species by Sandwich ELISA and Comparison with 16S rRNA Gene-Based NGS
Bacterial counts in three human fecal samples were measured using sandwich ELISA with combinations of anti-gut bacterial antibodies. Relative abundances obtained by sandwich ELISA were compared with those obtained from 16S rRNA gene amplicon sequencing via NGS1).
- Combination of antibodies in ELISA
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(1) P. vulgatus
Capture Antibody: PV-L1A6-117K2
Detection Antibody: PV-L2B7-117K1(2) F. duncaniae
Capture Antibody: FD-S2D3-18K1
Detection Antibody: FD-L4F6-18K2(3) S. copri
Capture Antibody: SC-S10C3-49K1
Detection Antibody: SC-S10C3-49K1
Quantification of Gut Bacterial Counts by Sandwich ELISA
- Phocaeicola vulgatus
- Faecalibacterium duncaniae
- Segatella copri
Comparison with 16S rRNA Gene-Based NGS
- Phocaeicola vulgatus
- Faecalibacterium duncaniae
- Segatella copri
Flow Cytometry
After fixation with Farmer’s fixative (ethanol:acetic acid = 7:3), the bacteria were centrifuged at 13,040 x g for 2 minutes at 4 °C. Next, 100 μL of the bacterial suspension was mixed with 1 μg of anti-gut bacterial antibody in PBS-T containing 1% BSA and incubated on ice for 1 hour. After washing, FITC-conjugated anti-mouse IgG was added, and the sample was incubated for 30 minutes on ice in the dark. The stained bacteria were then washed and analyzed by flow cytometry1).
Anti-S. copri
Western Blotting
Segatella copri JCM13464 was collected by centrifugation at 10,000 x g for 5 minutes. Two micrograms of protein were separated by SDS-PAGE, transferred to a PVDF membrane, and blocked. Immunodetection was performed using 25–50 ng/mL of anti-gut bacterial antibody and HRP-conjugated mouse IgG1).
References
- Yoshii, K. et al.: Sci. Rep., 15(1), 1(2025).
Establishment of enterotype-specific antibodies for various diagnostic systems
FAQ
About antibody
- Does this product react with Segatella species other than Segatella copri?
- Cross-reactivity has only been confirmed for Segatella copri used in the immune response; cross-reactivity with other bacterial species within the same genus remains unknown.
Overview / Applications
Property
Manufacturer Information
Alias
- Anti Prevotella, Monoclonal Antibody (SC-S10C3-49K1)
For research use or further manufacturing use only. Not for use in diagnostic procedures.
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