iMatrix-511Silk
- Manufacturer :
- Matrixome
- Storage Condition :
- Keep at 2-10 degrees C.
- Structural Formula
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175ug x6
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In stock in Japan |
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※Check availability in the US with the distributor.
Document
What is laminin 511?
Laminin constitutes a part of the extracellular matrix of the basement membrane of animal cells and is involved in the processes of cell adhesion and proliferation. iMatrix-511 Silk is a recombinant protein having the same sequence as laminin 511-E8 fragment. It is a cell culture substrate that promotes adhesion and extension of a variety of cells (ex. ES cells, iPS cells and mesenchymal stem cells).
A collaborative study led by Osaka University and Kyoto University demonstrated that iMatrix-511 Silk also facilitates culturing of iPS and ES cells, which was considered very difficult, in a safe and highly efficient manner.
- Facilitates cell culture preparation
- Can be used for culturing various types of cells
- Promotes proliferation of cells with high viability and efficiency regardless of the state of dissociation
- Low risk of contamination as the product is a recombinant protein derived from CHO-S cells
- No need to dissolve as it is available as a solution; just needs to be diluted
Laminin 511 constitutes of α5-chain, β1-chain, and γ1-chain. Although laminin 511-E8 is a fragment, it has the ability to bind to α6β1 integrin as with full-length laminin.
※iMatrix-511 Silk is a licensed technology of Osaka University and Kyoto University, and is produced and sold by Nippi Inc..
How to use
- Dilute iMatrix-511 solution with PBS (-) and put onto the cell culture surface at a final concentration of 0.1-1.5 µg/cm2.
※The optimal concentration for coating varies with the type of cells, cell lines, and the culture medium used. For the first time usage, start with the final concentration of 0.5 µg/cm2 and determine the optimal condition for coating. - Incubate in room temperature for 3 hours and discard the solution.
- Put cells and culture medium to start cell culture.
Feeder-free, single-cell passaging is possible when culturing ES/ iPS cells.
Example
Epidermal cells and vascular endothelial cells were cultured with iMatrix-511 solution for 0.5 and 1 hour, respectively.
(a) Epidermal cells, 0.5 h-culture
Left (Non-Cort): Only few cells have adhered.
Right (iMatrix): Many of the cells have extended and adhered.
(b) Vascular endothelial cells, 1 h-culture
Left (Non-Cort): Most cells are round, while only few have adhered.
Right (iMatrix): Only few cells are round, while most cells have extended well.
Differences between iMatrix-511 and iMatrix-511 silk
| iMatrix_511 | iMatrix-511 silk | |
|---|---|---|
| Source of the material (Recombinant protein expression system) |
Recombinant CHO-S cell | Transgenic silkworms |
| Raw material | CHO-S cell culture supernatant | Silkworm cocoon |
| Product grade | Experimental＊ | Experimental |
| Transgene | Human laminin 511-E8 fragment | |
| Purity | ≥ 95 % | |
| Concentration | 0.5 mg/mL | |
| Dissociation constant | ≤ 10 nM | |
| Expiration date | 2 years after the production date | |
| Use for iPS cell culture | iPS cell production and maintenance are possible when used at 0.5 μg/cm2 for coating | |
＊Clinical-grade product is also available
Overview / Applications
| Outline | Sodium Chloride Disodium Hydrogen Phosphate Dipotassium Hydrogen Phosphate |
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Property
Manufacturer Information
Alias
For research use or further manufacturing use only. Not for use in diagnostic procedures.
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