Highly Cross-linked Polystyrene Support "Aminolinker PS"
The Highly Cross-linked Polystyrene Supports provides significant benefits for oligonucleotide synthesis. It delivers high coupling efficiency and excellent reagent washing efficiency during the synthesis process, resulting in oligonucleotides of higher purity compared to other supports. Additionally, its superior physical strength minimizes damage during synthesis. Its low-swelling property ensures minimal volume change throughout the process, eliminating the need to take swelling into consideration when packing the support into reaction vessels. As a result, it can be used as easily as controlled pore glass (CPG).
"Aminolinker PS" is a solid support specifically designed for the chemical synthesis of oligonucleotides. It features a porous, low-swelling polystyrene matrix. The physical characteristics of the polystyrene resin--such as particle size, shape, and pore size--are precisely controlled. This support is provided in its native form, functionalized with an amino linker. Nucleosides or universal linkers can be conjugated to it for oligonucleotide synthesis. Bulk quantities and custom modifications tailored to your specifications are also available. Please contact us for further details.
<Solid-phase Oligonucleotide Synthesis>
<Structure and Morphology of Aminolinker PS>
Structure of Aminolinker PS
SEM image of PS resin
Appearance
Features
- Low-Swelling Polystyrene Support
Exhibits minimal swelling, resulting in high reagent washing efficiency. - High Yield
Achieves a high oligonucleotide yield per synthesis scale. - No Need to Consider Voids
Due to low swelling, void volume adjustment during column packing is unnecessary. - Free Modification Enabled by Amine-Terminated Linkers
The native amino linker type allows free modification of starting nucleosides and universal linkers. - Selectable Pore Size
Available pore sizes are 1000, 2000, and 3000 Å (4000 Å available as a customization). - Customizable Options Available
Supports bulk supply and flexible modification options.
Physical Properties
| Aminolinker PS 1000A | Aminolinker PS 2000A | Aminolinker PS 3000A | ||
|---|---|---|---|---|
| Product No. | 018-28981 | 015-29111 | 012-29121 | |
| Particle size | 53-100 μm | 53-100 μm | 53-100 μm | |
| Pore size | 1000Å (100 nm) | 2000Å (200 nm) | 3000Å (300 nm) | |
| Dry volume | 3.9 mL/g | 4.2 mL/g | 4.3 mL/g | |
| Swelling volume | Acetonitrile | 4.1 mL/g | 4.4 mL/g | 4.5 mL/g |
| Toluene | 4.1 mL/g | 4.5 mL/g | 4.6 mL/g | |
| THF | 4.2 mL/g | 4.5 mL/g | 4.6 mL/g | |
| Dichloromethane | 4.3 mL/g | 4.6 mL/g | 4.8 mL/g | |
| Reactive group | NH2 | NH2 | NH2 | |
| Amino loading | 40-60 μmol/g | 40-60 μmol/g | 40-60 μmol/g | |
Applications
Synthesis of oligonucleotides (T10 and T20) using Aminolinker PS 1000A in DMTr-Off mode
1) Synthesis of T10mer
| Solid Supports | UnyLinker™ | Crude Yield (%) |
Crude Purity (HPLC, area%) |
|||||
|---|---|---|---|---|---|---|---|---|
| Supplier | Materials | Pore Size (Å) | Substituent on N | Functional Loading (µmol/g) |
T9 <N-1> |
T10 <FLP> |
||
| 1 | FUJIFILM Wako | HCP (Low-Swelling Polymer) |
1000 | Ph | 49 | >90 | 5 | 92 |
| 2 | 2000 | 27 | >90 | 3 | 92 | |||
| 3 | 3000 | 29 | >90 | 4 | 90 | |||
| 4 | 4000 | 8 | >90 | 4 | 90 | |||
| C1 | Competitor A | CPG | 500 | Ph | 90 | 69 | 10 | 83 |
| C2 | 1000 | 48 | 71 | 7 | 87 | |||
| C3 | Competitor B | CPG | 1000 | Me | 45 | 67 | 2 | 93 |
| C4 | Competitor C | Swelling Polymer | Swelling Pore | Ph | 407 | 73 | 1 | 95 |
| C5 | Competitor D | 347 | 79 | 2 | 94 | |||
Note: These results were obtained from limited sample sizes and specific conditions
a) FUJIFILM Wako PS 1000Å
b) Competitor CPG 1000Å
Figure HPLC analysis of ONs released from T10-loaded (a) UnyLinker™ PS 1000Å (R=Ph) and (b) UnyLinker™ CPG 1000Å (R=Me) under Basic conditions. Basic condition: AMA at 60℃ for 1 h.
HPLC condition of Oligonucleotide
| Column size | : | Wakopak® Ultra C18-2 φ2.1 mm×100 mm (D) |
| Mobile phase | : | A) 100 mM TEAA aq., B) Acetonitrile |
| Gradient | : | 0-15 min B=5-15%, 15-20 min B=100%, 20-25 min B=5% |
| Flow rate | : | 0.3 mL/min |
| Temperature | : | 40℃ |
| Detection | : | UV260 nm |
| Injection vol. | : | 0.5 µL |
| Sample | : | DNA dT 10 mer, All PO |
2) Synthesis of T20mer
| Solid Supports | UnyLinker™ | Crude Yield (%) |
Crude Purity (HPLC, area%) |
|||||
|---|---|---|---|---|---|---|---|---|
| Supplier | Materials | Pore Size (Å) | Substituent on N | Functional Loading (µmol/g) |
T19 <N-1> |
T20 <FLP> |
||
| 1 | FUJIFILM Wako | HCP (Low-Swelling Polymer) |
1000 | Ph | 29 | >90 | 5 | 87 |
| 2 | 2000 | 19 | >90 | 4 | 83 | |||
| 3 | 3000 | 20 | >90 | 5 | 80 | |||
| 4 | 4000 | 8 | >90 | 5 | 84 | |||
| C1 | Competitor A | CPG | 500 | Ph | 90 | 73 | 12 | 80 |
| C2 | 1000 | 48 | 69 | 7 | 83 | |||
| C3 | Competitor B | CPG | 1000 | Me | 45 | 68 | 8 | 86 |
| C4 | Competitor C | Swelling Polymer | Swelling pore | Ph | 407 | 57 | 4 | 79 |
| C5 | Competitor D | 347 | 69 | 12 | 78 | |||
Note: These results were obtained from limited sample sizes and specific conditions
HPLC condition of Oligonucleotide
| Column size | : | Wakopak® Ultra C18-2 φ2.1 mm×100 mm (D) |
| Mobile phase | : | A) 100 mM HFIP + 8.6mM TEA aq., B) Acetonitrile |
| Gradient | : | 0-15 min B=1-9%, 15-20 min B=100%, 20-25 min B=1% |
| Flow rate | : | 0.3 mL/min |
| Temperature | : | 40℃ |
| Detection | : | UV260 nm |
| Injection vol. | : | 0.25 µL |
| Sample | : | DNA dT 20 mer, All PO |
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