UniWells™ Horizontal Co-Culture Plate

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UniWells™ Horizontal Co-Culture Plate is an ideal co-culture device that connects two wells laterally.
Horizontal connection provides easy viewing of cells in both wells simultaneously using various type of microscopes.
It is a versatile tool for studying cell-cell interactions such as transport, migration and invasion.

Features

  • Simultaneous observation using a time-lapse microscope
  • No more filter clogging
  • Cells are cultured under the same condition (used same material on the bottom)
  • Filters of any membrane types and pore sizes are usable
  • Both wells are independent (connect in a free combination)

Excellent point

Both cells can be cultured under the same medium volume

  • Conventional co-colture plate
    Medium volume in A is more than in B and cell secreted factors A are diluted in B.
  • UniWells™
    Medium volume are the same between A and B

 

Filter is prevented from being clogged by cells

  • Conventional co-colture plate
    The filter is clogged by cells cultured in the upper well, which leads to interfere with migration of cell secreted factors between the upper and lower wells.
  • UniWells™

    The filter is NOT clogged by cells.

Kit Contents

UniWells™ Horizontal Co-Culture Plate (384-14421)

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Main Body A ・・・2
Main Body B ・・・2
Cover ・・・4
Common Cover ・・・4
Adapter ・・・1
O-ring ・・・2
[Note] The filters are sold separately

Example of use – Uptake of exosomes -

UniWells™ Horizontal Co-Culture Plate enables the observation that exosomes derived from cell B permeate the filter and are uptaked by cell A.

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Comparison of permeability testing of exosome between conventional co-culture plate and UniWells™

  1. Cells were seeded to be the same cell numbers into the upper well in conventional co-culture plate and one side well in Uniwells™.
  2. Co-culture started after 24 hours from cell seeding.
  3. Exosome densities of cell side and medium side were analyzed.

Instructions for use

  • Connected use

    Wells are connected by fitting a filter, O-ring and cover.

    ※The filters are sold separately.

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    Connection methods are as below;

    A)Connect the wells cultured independently by aspirating the culture solution once.
    B)Connect first and increase the volume of the culture solution to achieve co-culture

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  • Single use

    Fit a common cover and cover to a well.

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    Use in a microscope

    Set wells to the adapter provided.

    ※The adapter is attached to the main unit.

Medium volume

Medium volume less than 400 μL is not shared between the wells.

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The maximum medium volume on each well is 1.8 mL.

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FAQ

What is UniWells™ made of ?

Main body and common cover are made of polystyrene and low density polyethylene, respectively. UniWells™ filter, which is sold separately, is made of polycarbonate.

Is Uniwells™ Sterilized ?

Uniwells™ is sterilized by electron beam sterilization. Do not sterilize it by autoclaving.

Are the main body surfaces coated with anything ?

No, they are not coated. Coat the main body surfaces with attachment matrixes as needed.

Product List

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UniWells™ Horizontal Co-Culture Plate

UniWells™ Filter 0.6 µm

UniWells™ Filter 0.03 µm

UniWells™ Adapter 96

Product content may differ from the actual image due to minor specification changes etc.

877-714-1920

Hours of Operation: 8:00 - 17:00 (EST)For other hours than the above, please contact us via the inquiry form.