UniWells™ Horizontal Co-Culture Plate
UniWells™ Horizontal Co-Culture Plate is an ideal co-culture device that connects two wells laterally.
The horizontal connection provides easy viewing of cells in both wells simultaneously using various types of microscopes.
It is a versatile tool for studying cell-cell interactions such as transport, migration, and invasion.
Features
- Simultaneous observation using a time-lapse microscope
- No more filter clogging
- Cells are cultured under the same condition (used same material on the bottom)
- Filters of any membrane types and pore sizes are usable
- Both wells are independent (connect in a free combination)
Excellent points
Both cells can be cultured under the same medium volume
-
Conventional co-culture plate
Medium volume in A is more than in B and cell secreted factors A are diluted in B. -
UniWells™
Medium volume are the same between A and B
Filter is prevented from being clogged by cells
-
Conventional co-culture plate
The filter is clogged by cells cultured in the upper well, which leads to interfering with the migration of cell secreted factors between the upper and lower wells. -
UniWells™
The filter is NOT clogged by cells.
Kit Contents
UniWells™ Horizontal Co-Culture Plate (384-14421)
| Main Body A | ・・・2 |
| Main Body B | ・・・2 |
| Cover | ・・・4 |
| Common Cover | ・・・4 |
| Adapter | ・・・1 |
| O-ring | ・・・2 |
| [Note] The filters are sold separately | |
Example of use – Uptake of exosomes -
UniWells™ Horizontal Co-Culture Plate enables the observation that exosomes derived from cell B permeate the filter and are uptaken by cell A.
Comparison of permeability testing of exosome between conventional co-culture plate and UniWells™
- Cells were seeded to be the same cell numbers into the upper well in conventional co-culture plate and one side well in Uniwells™.
- Co-culture started after 24 hours from cell seeding.
- Exosome densities of the cell side and medium side were analyzed.
Instructions for use
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Connected use
Wells are connected by fitting a filter, O-ring and cover.
※The filters are sold separately.
Connection methods are as below;
A)Connect the wells cultured independently by aspirating the culture solution once.
B)Connect first and increase the volume of the culture solution to achieve co-culture
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Single use
Fit a common cover and cover to a well.
Use in a microscope
Set wells to the adapter provided.
※The adapter is attached to the main unit.
Medium volume
|
A medium volume of less than 400 μL is not shared between the wells.  |
The maximum medium volume on each well is 1.8 mL.  |
FAQ
What is UniWells™ made of?
The main body and common cover are made of polystyrene and low density polyethylene, respectively. UniWells™ filter, which is sold separately, is made of polycarbonate.
Is Uniwells™ Sterilized?
Uniwells™ is sterilized by electron beam sterilization. Do not sterilize it by autoclaving.
Are the main body surfaces coated with anything?
No, they are not coated. Coat the main body surfaces with attachment matrixes as needed.
Product List
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UniWells™ Horizontal Co-Culture Plate
UniWells™ Filter
UniWells™ Filter Mix Set
UniWells™ Adapter 96
For research use or further manufacturing use only. Not for use in diagnostic procedures.
Product content may differ from the actual image due to minor specification changes etc.
If the revision of product standards and packaging standards has been made, there is a case where the actual product specifications and images are different.