Early Diagnosis of Kidney Diseases - CMIC HOLDINGS Co., Ltd. products -

L-FABP(L-type Fatty Acid Binding Protein)

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This is an ELISA kit to measure Urinary L-FABP (L-type Fatty Acid Binding Protein) by sandwich method with colorimetric analysis.

※For Research Use Only.Not for use in diagnostic procedures
※This products is developed by CMIC HOLDINGS Co.,Ltd.

What is urinary L-FABP?

L-FABP is a carrier protein of 14kDa excreted in the proximal tubule and plays a role in the intracellular transport of FFAs to mitochondria and/or peroxisomes for metabolism. Conventional markers are excreted in urine due to tissue damage in the glomerulus and renal tubule. But L-FABPs are excreted in urine due to ischemia or low capillary blood flow and oxidative stress on renal tubule before the progression of tissue damage. Thus, L-FABP is considered as useful biomarker for early diagnosis of renal disease accompanying tubular dysfunction.

Mechanism of expression

Mechanism of expression

Conventional markers are excreted in urine due to tissue damage in the glomerulus and renal tubule.

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Characteristics

  • Excellent in Sensitivity, Accuracy, and reproducibility
  • Measurement Sample:urine
  • Measurement wavelength:450 nm (reference wavelength 610 nm or over)
  • Test time:about 3 hours
  • Measurement method:enzyme immunometric assay (Sandwich ELISA Method)

Example data

The Change of urinary L-FABP in each drug-induced nephropathy model

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Test animal: 6 week old male SD rats Administrated medicine:

  • Gentamicin (200mg/kg, s.c.)
  • Cisplatin (4mg/kg, i.v)
  • Amphotelicin B (50mg/kg, i.p.)

Procedure

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For early diagnosis of diabetic nephropathy

The level of urinary L-FABP increased significantly according to the severity of diabetic nephropathy. Urinary L-FABP in the patients with normal albuminuria was significantly higher than in normal control subjects. Thus, urinary L-FABP is a useful biomarker for early diagnosis of diabetic nephropathy

Relationship between urinary L-FABP levels and progression of diabetic nephropathy

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Urinary L-FABP was measured in 147 patients who had diabetic nephropathy and was divided according to the disease stage. The average value and standard variation of each disease stage were calculated and shown in the graph to the right, together with the urinary L-FABP value of healthy volunteers.

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