Wakopak® Wakosil® 5Ph
Wakopak® Wakosil® 5Ph is a reverse-phase HPLC column using packing agent with phenyl group chemically bonded. Wakopak® Wakosil® 5Ph can use for samples with a broad polar range as same as HPLC column using TMS packing agent.
In particular, it shows specific separation for aromatic compounds.
Applications
Comparison of retention of each packing agent in neutral compounds
| Column Size Eluent Detection |
：4.6 mm × 150 mm ：CH3CN/H2O=60/40 (v/v) ：UV 254 nm |
|
Analysis example of o, m, p-Nitroanilines
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Column
Eluent
Flow Rate
Detection：Wakopak® Wakosil® 5Ph（4.6 mm × 250 mm）
：CH3CN/H2O/CH3COOH=45/54/1 (v/v/v)
：1.0 mL/min. at R.T.
：UV 254 nm
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Wakopak® Wakosil® 5Ph
For research use or further manufacturing use only. Not for use in diagnostic procedures.
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