Wakopak® Wakosil®-Ⅱ 5C18 HG, RS, AR
Wakopak® Wakosil®-II 5C18 HG, RS, AR is a column packed with a completely spherical, porous, silica gel-based packing agent. To this column, "Wakopak® Certificate of Analysis", which describes the specification and measured values for "properties of unmodified silica gel" and "characteristics of packing agents after ODS modification" to support of analytical validation and assuring reproducibility within the specifications, is attached.
Features
- High-purity silica gel is used and is optimal for the analysis of metal coordination compounds
- Endcapping treatment is optimal for sharp separation of basic compounds
- High resolution / high number of theoretical plates
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Metal Coordination Compounds Basic Compounds
Certificate of Analysis
Application
Analysis of Scopolamine and Atropine
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Sample
Column size
Eluent
Flow Rate
Detection:1) Scopolamine HBr
2) Atropine Sulfate
3) Brucine (IS)
:4.6 mm x 150 mm
:CH3CN/50 mM KH2PO4, 1% Triethylamine (pH 3.5 ← H3PO4)
=10/90 (v/v)
:1.0 mL/min.
:UV 210 nm
Wakopak® Wakosil®-Ⅱ5C18 HG
Features
Wakopak® Wakosil®-II 5C18 HG uses a packing agent obtained by converting highly pure silica gel to monomeric ODS and endcapping. It is suitable for the analysis of basic compounds / metal coordination compounds. Particularly, this product features a high number of theoretical plates. The column efficiency is as high as approximately 15,000 plates at a column size of 4.6 mm × 150 mm, and good results, which have not been obtained with conventional columns, are obtained.
Analysis of Metal Coordination Compounds
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Column
Eluent
Flow Rate
Detection:Wakopak® Wakosil®-Ⅱ 5C18 HG (4.6 mm x 150 mm)
:CH3CN/20 mM K2HPO4 (pH 7.6) = 40/60 (v/v)
:1.0 mL/min. at 30℃
:UV 254 nm 0.16 AUFS
Analysis of Alkylphenols
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Column
Eluent
Flow Rate
Detection:Wakopak® Wakosil®-Ⅱ 5C18 HG (4.6 mm x 250 mm)
:CH3OH/H2O = 60/40 (v/v)
:1.0 mL/min. at 40℃
:UV 225 nm
Analysis of 13 Type of Catecholamine Metabolites
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Column
Eluent
Flow Rate
Detection:Wakopak® Wakosil®-Ⅱ 5C18 HG (4.6 mm x 150 mm)
:CH3OH/0.1M KH2PO4 , H3PO4 (pH3.9) = 10/90 (v/v) containg SOS 240 mg/L
:1.0 mL/min. at 35℃
:ECD + 800 mV, 50 nAFS
Wakopak® Wakosil®-Ⅱ5C18 RS
Features
By improving ODS treatment and endcapping, high resolution / high retention capability are also achieved in aqueous eluents, and in particular, retention is in a balanced manner in mixed samples with a wide range of polarities. In addition, thorough endcapping of the residual silanol groups enables sharp analysis of basic compounds without tailing.
Procainamide and Phenol Analysis
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Sample
Column
Eluent
Flow Rate
Detection:1) Procainamide Hydrochloride
2) Phenol
:Wakopak® Wakosil®-Ⅱ 5C18 RS (4.6 mm x 150 mm)
:CH3OH/20 mM K2HPO4, (pH 7.6) = 40/60 (v/v)
:1.0 mL/min. at 30℃
:UV 254 nm
Analysis of Water-soluble Vitamins
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Sample
Column
Eluent
Flow Rate
Detection:1) L(+)-Ascorbic acid(V.C)
2) Nicotinic acid
3) Nicotinamide
4) Pyridoxine・HCl (V.B1)
5) Caffein
6) Thiamine (V.B1)
7) d-Biotin (B.H)
8) Riboflavin (V.B2)
:Wakopak® Wakosil®-Ⅱ 5C18 RS (4.6 mm x 150 mm)
:CH3OH/0.1% H3PO4 , 5 mM SHS* = 10/90 (v/v)
:1.0 mL/min. at 40℃
:UV 210 nm. 0.16 AUFS
*: 1-Hexanesulfonic Acid Sodium Salt
Wakopak® Wakosil®-Ⅱ5C18 AR
Features
Wakopak® Wakosil®-II 5C18 AR uses a polymeric ODS packing agent. This packing agent is a very durable, and even when the mobile phase is acidic (pH1.4), ODS is not readily hydrolyzed. It is useful for the separation of peptides and structural isomers based on the characteristics of polymeric ODS. In addition, separation characteristics may differ from those of typical monomeric ODS silica gel and may show a very specific behavior.
Analysis of Endothelins (ETs)
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Column
Eluent
Flow Rate
Detection:Wakopak® Wakosil®-Ⅱ 5C18 AR (4.6 mm x 150 mm)
:A) CH3CN/0.1%TFA = 20/80 (v/v)
B) CH3CN/0.1%TFA = 50/50 (v/v)
0-15 min. B conc. 30-70%
:1.0 mL/min. at 40℃
:UV 220 nm
Analysis of Enkephalins
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Column
Eluent
Flow Rate
Detection:Wakopak® Wakosil®-Ⅱ 5C18 AR (4.6 mm x 150 mm)
:A) H2O 0.1% TFA
B) CH3CH 0.1% TFA
0→25 min. B conc. 10-60%
25→30 min. B conc. 60%
:1.0 mL/min. at 40℃
:UV 220 nm 0.256 AUFS.
Analysis of Polycyclic Aromatic Hydrocarbons
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Column
Eluent
Flow Rate
Detection:Wakopak® Wakosil®-Ⅱ 5C18 AR (4.6 mm x 250 mm)
:A) CH3CN B)H2O
0→3 min. A conc. 50%
3→10 min. A conc. 50→100%
:1.5 mL/min. at R.T.
:UV 254 nm 0.128 AUFS.
Resistance Data
Acid resistance data |
After passing 1.0% trifluoroacetic acid solution (pH1.4) to each column, |
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Alkaline resistance data |
Passing the mobile phase continuously to each packed column at 40°C. |
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Wakopak® Wakosil®-Ⅱ 5C18 HG
Wakopak® Wakosil®-Ⅱ 5C18 RS
Wakopak® Wakosil®-Ⅱ 5C18 AR
For research use or further manufacturing use only. Not for use in diagnostic procedures.
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