Wakopak® Wakosil®-Ⅱ 3C18 HG, RS, AR
- Completely porous, spherical packing agent with a diameter of 3 μm.
- The number of theoretical plates and resolution are improved compared with the 5 μm packing agent (≥100,000 theoretical plates/m). Therefore, it is possible to shorten the column.
- Line-up three types, HG, RS, and AR, as in the case of the 5C18 Series, and facilitate the migration of analytical conditions.
- Solvent usage is reduced, thus economizing on resources.
- Sample microsizing and high-sensitivity analysis are possible.
- Can be used in LC/MS.
Applications
Investigation of column shortening (analysis of preservative (paraben))
-
Sample
Eluent
Flow Rate
Detection：1）Methyl p-Hydroxybenzoate
2）Ethyl p-Hydroxybenzoate
3）Isopropyl p-Hydroxybenzoate
4）n-Propyl p-Hydroxybenzoate
5）sec-Butyl p-Hydroxybenzoate
6）Isobutyl p-Hydroxybenzoate
7）n-Butyl p-Hydroxybenzoate
：CH3OH/5 mM Citrate buffer（pH4.0）
：0.8 mL/min. at 40℃
：UV 270 nm
Features of semi-micro columns
Semi-micro columns with an inside diameter of 1.0 to 2.0 mm can be used at a lower flow rate than a general-purpose column to reduce the amount of solvent used. And semi-micro columns are suitable for use in LC/MS. In addition, analysis is possible with a small amount of sample by improved sensitivity. For example, when columns with inside diameters of 2.0 mm and 4.6 mm are compared, the ratio of cross-sectional areas is approximately 1/5; therefore, the sensitivity is increased by a factor of approximately 5, and the amount of solvent consumed is approximately 1/5.
Data comparison between 3 mm and 4.6 mm internal diameter columns
A general-purpose column with an internal diameter of 3.0 mm achieves a solvent consumption of approximately 1/2 and approximately double the sensitivity with the equipment available presently, in contrast to the equipment responding to the semi-micro column with the detector cell volume.
Example of shortened analysis time (Veterinary Drug Mixture Standard Solution PL-2-1: 24 species mixture, 20 μg/mL each of methanol solution)
Column: Wakopak® Wakosil®-Ⅱ 3C18 HG
positive mode 23 ingredients 0.1 ppm
negative mode 1 ingredients 0.1 ppm
HPLC conditionss
| Column Eluent |
：Wakopak® Wakosil®-Ⅱ 3C18 HG ：A）0.1％ HCOOH in H2O* B）0.1％ HCOOH in CH3CN** |
|||||||||||||||
| Time Program |
|
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| Flow Rate Injection Vol. |
：0.2 mL/min. at 40℃ ：0.1 ppm, 4 μL (3.0 x 150 mm), 3 μL (2.0 x 100 mm) |
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| * ** |
Formic Acid (abt. 99%) for LC/MS (Code No. 067-04531) Ultrapure Water for LC/MS (Code No. 214-01301) 0.1 vol% Formic Acid-Acetonitrile (Code No. 062-04721) |
MS/MS conditions
ESI, MRM
| Ion Spray Voltage Temperature Curtain Gas Collision Gas Ion Source Gas 1 Ion Source Gas 2 System |
：5500 V (pos), -4500 V (neg) ：400℃ (po9), 500℃ (neg) ：20 ：6 ：50 ：80 ：3200Q TRAP (ABI) |
Veterinary Drug Mixture Standard Solution PL-2-1: 24 species mixture
| Peak No. |
Analyte Peak Name | Analyte Mass Ranges (amu) |
Analyte Retention Time (min) |
|||
|---|---|---|---|---|---|---|
| Precursor | Product | Mode | 3 x 150 mm | 2 x 100 ｍｍ | ||
| 1 | 5-Propylsulfonyl-1H-benzimidazole-2-amine | 240.1 | 133.2 | + | 11.2 | 4.7 |
| 2 | Levamisole | 207.1 | 91.2 | + | 11.3 | 4.7 |
| 3 | Thiabendazole | 202.1 | 175.1 | + | 11.8 | 4.9 |
| 4 | Trimethoprim | 291.1 | 230.3 | + | 12.0 | 4.9 |
| 5 | Sulfadiazine | 251.1 | 92.2 | + | 12.3 | 5.0 |
| 6 | Ormetoprim | 275.2 | 123.1 | + | 12.5 | 5.1 |
| 7 | Sulfathiazole | 256.0 | 156.0 | + | 12.8 | 5.2 |
| 8 | Sulfapyridine | 250.0 | 156.2 | + | 13.2 | 5.3 |
| 9 | Sulfamerazine | 265.1 | 92.2 | + | 13.8 | 5.5 |
| 10 | Thiamphenicol | 355.9 | 308.0 | + | 14.4 | 5.7 |
| 11 | Sulfadimidine | 279.1 | 92.1 | + | 15.0 | 5.9 |
| 12 | Sulfamethoxypyridiazine | 281.1 | 156.1 | + | 15.0 | 5.9 |
| 13 | Sulfamonomethoxine | 281.1 | 156.1 | + | 16.1 | 6.2 |
| 14 | Sulfachlorpyridazine | 286.1 | 157.1 | + | 16.7 | 6.4 |
| 15 | Sulfamethoxazole | 254.0 | 156.0 | + | 17.5 | 6.6 |
| 16 | Sulfadoxine | 311.1 | 156.1 | + | 17.4 | 6.6 |
| 17 | Ethopabate | 238.2 | 136.0 | + | 19.3 | 7.2 |
| 18 | Sulfaquinoxaline | 301.0 | 156.2 | + | 19.4 | 7.2 |
| 19 | Sulfadimethoxine | 311.1 | 156.2 | + | 19.4 | 7.2 |
| 20 | Sulfanitran | 336.1 | 134.1 | + | 21.9 | 7.9 |
| 21 | β-Trenbolone | 271.2 | 165.3 | + | 23.2 | 8.3 |
| 22 | α-Trenbolone | 271.2 | 165.2 | + | 23.7 | 8.5 |
| 23 | Melengestrol Acetate | 397.2 | 279.4 | + | 31.3 | 10.7 |
| 1 | Zeranol | 321.1 | 277.2 | - | 24.2 | 8.7 |
Veterinary Drug Mixture Standard Solution PL-2-1 (Code No. 227-01691)
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Wakopak® Wakosil®-Ⅱ 3C18 HG
Wakopak® Wakosil®-Ⅱ 3C18 RS
Wakopak® Wakosil®-Ⅱ 3C18 AR
For research use or further manufacturing use only. Not for use in diagnostic procedures.
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