Wakopak® Wakosil® C18 Series
Wakopak® Wakosil® series is a column packed with a completely spherical, porous, silica gel-based packing agent for HPLC. Wakopak® Wakosil® series assortment focuses on columns of different separation modes, especially reverse-phase distribution ODS packing agents, Wakopak® Wakosil® C18 series.
Features
High-endcapping type
ODS bond and endcapping treatments are performed FUJIFILM Wako’s unique surface treatment technology. Excellent peak shape and reproducibility are obtained for various samples, ranging from acidic to basic.
- Wakopak® Wakosil® C18 type (5C18, 7C18, 10C18)
It is a high-retention capability/high-resolution type with a carbon content of 20%, which is particularly effective for samples of high polarity and weak retention. - Wakopak® Wakosil® 5C18 AR
It is a polymeric ODS packing agent that minimizes ODS leakage and shows excellent durability, even under acidic mobile phases and water-rich conditions. - Wakopak® Wakosil® C18-200 type (5C18-200, 10C18-200)
The carbon content is as low as 12%, and rapid analysis can be performed. However, the resolution is almost equivalent to that of the Wakopak® Wakosil® C18 type, and high resolution is maintained.
Endcapping type
- Wakopak® Wakosil® 5C18T, 5C18-200T
Endcapping is performed after ODS bond, but a small amount of silanol group remains. It is useful when using reverse-phase distribution and slight silanol adsorption for separation, such as with glycosides and medical products. For neutral samples, this product exhibits retention characteristics similar to those of the high-endcapping type.
Non-endcapping type
- Wakopak® Wakosil® 5C18N, 5C18-200N
The silica gel surface is maximally ODS-bonded, but endcapping is not performed. It is a packing agent with more silanol group adsorptive property than the Wakopak® Wakosil®-T type.
In addition, the residual silanol content of Wakopak® Wakosil® 5C18N is higher than that of 5C18-200N due to the physical properties of silica gel, resulting in higher adsorptive effects.
It is useful for separation analysis of positional isomers or compounds with similar structures.
Comparison of endcapping test
Applications
Bufosteroid analysis
Phthalic acid analysis
Analysis of leukotrienes
Product List
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Wakopak® Wakosil® 5C18
Wakopak® Wakosil® 5C18T
Wakopak® Wakosil® 5C18N
Wakopak® Wakosil® 7C18
Wakopak® Wakosil® 10C18
Wakopak® Wakosil® 5C18AR
Wakopak® Wakosil® 5C18-200
Wakopak® Wakosil® 5C18-200T
Wakopak® Wakosil® 5C18-200N
Wakopak® Wakosil® 10C18-200
For research use or further manufacturing use only. Not for use in diagnostic procedures.
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