Glucagon ELISA kit Wako (Sandwich method)
Glucagon is a peptide hormone consisting of 29 amino acids secreted from the α cells of pancreatic islets of Langerhans. The main physiological function of glucagon is to decompose glycogen in the liver into glucose, increase blood glucose level, and keep glucose level constant with insulin.
- High specificity to glucagon
- High sensitivity(0.3pmol/L-)
- Small sample volume(10μL-)
This item is glucagon sandwich ELISA kit using 2 kinds of monoclonal antibodies. One reacts to the N-terminal of glucagon and the other reacts to the C-terminal.
Glucagon and glucagon-like peptide
Glucagon and glucagon-like peptides are produced by proglucagon cleavage. Several glucagon-like peptides containing similar amino acid sequence as glucagon have been reported.
Name Cross-reactivity（%） Glicentin(1-69) (human) 0.68 Glicentin(1-69) (rat) 0.96 Glicentin(1-69) (mouse) 0.97 Glicentin(1-61) (human) 0.95 Glicentin(1-61) (rat) not confirmed Glicentin(1-61) (mouse) not confirmed Oxyntomodulin (human, rat and mouse) 0.64 Mini-glucagon (human, rat and mouse) N.D.
Name Cross-reactivity（%） GLP-1(7-36) NH2 (human, rat and mouse) N.D. GLP-1(9-36) NH2 (human, rat and mouse) N.D. GLP-2 (human) N.D. GLP-2 (rat) N.D. GLP-2 (mouse) N.D. GIP (human) N.D. GIP (rat) N.D. GIP (mouse) N.D.
|Standard curve||2.2~143.6 pmol/L(7.8~500pg/mL)|
|Sensitivity||0.3 pmol/L (1.08 pg/mL)|
|Sample Type||Serum(Human, rat and mouse）
Plasma(Human, rat and mouse）
Cell culture supernatants
|Assay time||About 20 hour|
Correlation with competitor's products
Human serum (n=78)
Assayed according to the instruction manuals.
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