GLP-1
GLP-1 is thegastrointestinal peptide hormone secreted from intestinal L cells, and shows the hypoglycemic action through the enhancement of insulin secretion and the suppression of glucagon secretion by the sugar stimulus. The report has been issued that GLP-1 is useful for the treatment of type 2 diabetes as the new therapeutic agent for diabetes.
This product is ELISA kit measurable with a high sensitivity of GLP-1 in the plasma of mouse and rat used with a highly specific GLP-1 antibody. It has more than 10 times sensitivity compared to the conventional product (Rat GLP-1 ELISA Kit wako).
Since this kit reacts to both the active form of GLP-1 (7-36 amide) and the inactive form of GLP-1 (9-36 amide), it is suitable for experimental system measuring the total amount of GLP-1.
Features
- High sensitivity
The range of calibration curve : 0.94～30 pM
※In order to avoid the effect by the inhibitor in the blood, we recommend a five-fold dilution of the specimen, the substantial measurement range is 4.7 ~ 150pM. - Measurable in a short period of time.
Measurement time : 4 hours - A necessary amount of a specimen is small.
A necessary amount of a specimen : 10 μL
Comparison with conventional products
- Sensitivity more than 10 times
- Measurement time less than 1/4
- A necessary amount of a specimen 1/3
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Examples of measurement
Mouse plasma
| Mouse No. | Measured value (pM) | C.V value | Remarks |
|---|---|---|---|
| 1 | 31.4 | 3.1 | Fasted mouse, 8-week-old ♂ ※ Collected the blood after 15 hours fast |
| 2 | 16.3 | 2.9 | |
| 3 | 17.2 | 3.7 | |
| 4 | 20.8 | 3.1 | |
| 5 | 27.1 | 0.3 | |
| 6 | 39.6 | 2.6 | Sugar-administered mouse, 8-week-old ♂ ※ After the fast, administered glucose (2g / kg), then 10 minutes later, collected the blood. |
| 7 | 15.9 | 4.3 | |
| 8 | 9.4 | 3.8 | |
| 9 | 8.09 | 4.4 | |
| 10 | 13.9 | 1.8 |
→ A data of high reproducibility with a small C.V value was obtained.
Example of calibration curve
Kit performance
| The range of calibration curve | 0.94～30 pM |
|---|---|
| The substantial measurement range | 4.7 ~ 150 pM (converted as 5-fold dilution) |
| A necessary amount of a specimen | 10μL |
| Measurement sample | Plasma |
| Measurement time | 4 hours |
| Species crossing | Mouse, Rat |
| Simultaneous reproducibility | < 10% |
| Day difference reproducibility | < 10% |
Kit Components
| 1) Antibody-coated plate (8 x 12) wells/plate | 1 plate |
| 2) GLP-1 Standard Solution (30 pM) | 400 μL |
| 3) Buffer solution | 60 mL |
| 4) Biotinylated Anti GLP-1 Antibody Solution | 100 μL |
| 5) Peroxidase-conjugated streptavidin Soln. | 100 μL |
| 6) TMB Solution | 12 mL |
| 7) Stop Solution | 12 mL |
| 8) Wash Solution (x 10) | 100 mL |
| 9) Plate Seal | 4 seals |
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Total GLP-1 Measurement Kit
Active GLP-1 Measurement Kit
For research use or further manufacturing use only. Not for use in diagnostic procedures.
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