GLP-1 ELISA Kit Wako, High Sensitive
GLP-1 is thegastrointestinal peptide hormone secreted from intestinal L cells, and shows the hypoglycemic action through the enhancement of insulin secretion and the suppression of glucagon secretion by the sugar stimulus. The report has been issued that GLP-1 is useful for the treatment of type 2 diabetes as the new therapeutic agent for diabetes.
This product is ELISA kit measurable with a high sensitivity of GLP-1 in the plasma of mouse and rat used with a highly specific GLP-1 antibody. It has more than 10 times sensitivity compared to the conventional product (Rat GLP-1 ELISA Kit wako).
Since this kit reacts to both the active form of GLP-1 (7-36 amide) and the inactive form of GLP-1 (9-36 amide), it is suitable for experimental system measuring the total amount of GLP-1.
- High sensitivity
The range of calibration curve : 0.94～30 pM
※In order to avoid the effect by the inhibitor in the blood, we recommend a five-fold dilution of the specimen, the substantial measurement range is 4.7 ~ 150pM.
- Measurable in a short period of time.
Measurement time : 4 hours
- A necessary amount of a specimen is small.
A necessary amount of a specimen : 10 μL
Comparison with conventional products
- Sensitivity more than 10 times
- Measurement time less than 1/4
- A necessary amount of a specimen 1/3
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Examples of measurement
|Mouse No.||Measured value (pM)||C.V value||Remarks|
|1||31.4||3.1||Fasted mouse, 8-week-old ♂
※ Collected the blood after 15 hours fast
|6||39.6||2.6||Sugar-administered mouse, 8-week-old ♂
※ After the fast, administered glucose (2g / kg), then 10 minutes later, collected the blood.
→ A data of high reproducibility with a small C.V value was obtained.
Example of calibration curve
|The range of calibration curve||0.94～30 pM|
|The substantial measurement range||4.7 ~ 150 pM (converted as 5-fold dilution)|
|A necessary amount of a specimen||10μL|
|Measurement time||4 hours|
|Species crossing||Mouse, Rat|
|Simultaneous reproducibility||< 10%|
|Day difference reproducibility||< 10%|
|1) Antibody-coated plate (8 x 12) wells/plate||1 plate|
|2) GLP-1 Standard Solution (30 pM)||400 μL|
|3) Buffer solution||60 mL|
|4) Biotinylated Anti GLP-1 Antibody Solution||100 μL|
|5) Peroxidase-conjugated streptavidin Soln.||100 μL|
|6) TMB Solution||12 mL|
|7) Stop Solution||12 mL|
|8) Wash Solution (x 10)||100 mL|
|9) Plate Seal||4 seals|
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Total GLP-1 Measurement Kit
Active GLP-1 Measurement Kit
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