PYY
PYY is a gastrointestinal peptide hormone consisting of 36 amino acid residues. It is mainly released from endocrine cells localizing in the intestinal tract after food ingestion, and inhibits appetite through the vagus nerve and blood. The ELISA kit is used for the quantitative determination of mouse/rat PYY in plasma and serum samples.
Performance
| Standard curve range | 0.15～12.5 ng/mL |
| Test Time | about 20 hours |
| Samples | Mouse/Rat Serum, Plasma |
| Sample volume | 25μL |
| Reproducibility | Intra-assay CV(%) 3.1-9.8 Inter-assay CV(%) 4.2-14.2 |
| Cross-reactivity | Specific to Mouse,Rat No cross reactivity to Human PYY |
Kit Components
| 1) Antibody-coated plate | 1 plate |
| 2) Mouse/Rat PYY Standard | 12.5 ng |
| 3) Biotinylated Mouse/Rat PYY | 1 vial |
| 4) Anti Mouse/Rat PYY , Rabbit | 8.5 mL |
| 5) HRP Labeled Streptavidin Solution | 12 mL |
| 6) Enzyme Substrate Solution | 12 mL |
| 7) Stoppig Solution | 12 mL |
| 8) Buffer Solution | 25 mL |
| 9) Washing Solution (concentrated) | 50 mL |
| 10) Adhesive Foil | 3 seals |
Reference
- Watanabe, K. et al.: PLoS One., 13, e0202083(2018)
- Nakatani, A. et al.: Biochem. Biophys. Res. Commun., 501, 955(2018)
- Miyamoto, J. et al.: PLoS One., 13, e0196579(2018)
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