Frozen Neuron Series
The products in this series are frozen brain tissues collected from rat and mouse embryos. They reduce the time needed to prepare the animal models. Use our neuron dissociation solution (code No. 291-78001,297-78101) to dissociate the tissues, and use our neuron culture medium (code No. 148-09671) to culture cells collected from the tissues.
Features
- No need for the preparation of rat and mouse models that are otherwise time-consuming.
- Enables stable dissociation and culture of neurons by combining with neuron culture medium and neuron dissociation solution.
Protocol
Dispersion and Isolation of Frozen Neurons
The products in our frozen neuron series were thawed, and our dissociation solution was used to disperse and isolate the cells. Cell viability was calculated as % of total cells.
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Product name ① Hippocampus, from mouse
(embryonic day 16)Total number of cells(cells/vial) 4.32 x 105 Total number of viable cells(cells/vial) 3.83 x 105 Cell viability(%) 89 ② Cerebral cortex, from mouse
(embryonic day 15)Total number of cells(cells/vial) 6.64 x 106 Total number of viable cells(cells/vial) 6.09 x 106 Cell viability(%) 92 ③ Hippocampus, from rat
(embryonic day 19)Total number of cells(cells/vial) 1.37 x 106 Total number of viable cells(cells/vial) 1.24 x 106 Cell viability(%) 90 ④ Cerebral cortex, from rat
(embryonic day 17)Total number of cells(cells/vial) 1.32 x 107 Total number of viable cells(cells/vial) 1.17 x 107 Cell viability(%) 89 ⑤ Cerebral striatum, from rat
(embryonic day 17)Total number of cells(cells/vial) 2.73 x 106 Total number of viable cells(cells/vial) 2.58 x 106 Cell viability(%) 95
The data demonstrated that neurons were isolated from frozen samples with approximately 90% viability.
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For research use or further manufacturing use only. Not for use in diagnostic procedures.
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