Having difficulties preparing rats and mice for cell isolation?

Frozen Neuron Series

The products in this series are frozen brain tissues collected from rat and mouse embryos. They reduce the time needed to prepare the animal models. Use our neuron dissociation solution (code No. 291-78001,297-78101) to dissociate the tissues, and use our neuron culture medium (code No. 148-09671) to culture cells collected from the tissues.

Features

  • No need for the preparation of rat and mouse models that are otherwise time-consuming.
  • Enables stable dissociation and culture of neurons by combining with neuron culture medium and neuron dissociation solution.

Protocol

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Dispersion and Isolation of Frozen Neurons

The products in our frozen neuron series were thawed, and our dissociation solution was used to disperse and isolate the cells. Cell viability was calculated as % of total cells.

  • 00823_img02.png
  • Product name
    Hippocampus, from mouse
    (embryonic day 16)
    Total number of cells(cells/vial) 4.32 x 105
    Total number of viable cells(cells/vial) 3.83 x 105
    Cell viability(%) 89
    Cerebral cortex, from mouse
    (embryonic day 15)
    Total number of cells(cells/vial) 6.64 x 106
    Total number of viable cells(cells/vial) 6.09 x 106
    Cell viability(%) 92
    Hippocampus, from rat
    (embryonic day 19)
    Total number of cells(cells/vial) 1.37 x 106
    Total number of viable cells(cells/vial) 1.24 x 106
    Cell viability(%) 90
    Cerebral cortex, from rat
    (embryonic day 17)
    Total number of cells(cells/vial) 1.32 x 107
    Total number of viable cells(cells/vial) 1.17 x 107
    Cell viability(%) 89
    Cerebral striatum, from rat
    (embryonic day 17)
    Total number of cells(cells/vial) 2.73 x 106
    Total number of viable cells(cells/vial) 2.58 x 106
    Cell viability(%) 95

The data demonstrated that neurons were isolated from frozen samples with approximately 90% viability.

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Medium

Dispersion solution for nerve cells

Cryopreservation solution

NS Basal Medium / NS Supplement

Product content may differ from the actual image due to minor specification changes etc.

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