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For coating cell culture vessel surfaces

Laminin Solution

Laminins are a major basement membrane component of most animal tissues. . In cell culture, laminins have been used for coating the surfaces of cell culture vessels. It is widely known that laminin-coated surfaces enhanced attachment to certain types of cells (primarily epithelial cells, neurons, hepatocytes, and muscle cells).

Overview

  • Concentration: 0.5 mg/ml (the value obtained with the first lot)
  • Formulation: 50 mM Tris-HCl (pH 7.4), 150 mM NaCl.
  • Sterility: Filtered through a 0.2 μm pore size membrane.
  • Storage Conditions: Store at –80°C. (Stable at –20ºC for one year.)

Product Validation

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The laminin stock solution was diluted with D-PBS (calcium- and magnesium-free) to the concentrations indicated in the figure. Diluted laminin solutions were placed in 24-well plates (0.3 ml/well) and incubated overnight at 37℃. Following incubation, the laminin solutions were removed by aspiration and the wells were blocked with BSA. TIG-3 cells were then plated at a density of 5×104 cells/ml and were allowed to attach to the wells for 90 min at 37°C. Subsequently, unattached cells were removed by washing. The remaining adherent cells were harvested by treatment with trypsin-EDTA and used for cell number quantification.

Frequently Used Cell Types

  • Epithelial cells
  • Nerve cells
  • Liver cells
  • Muscle cells

Instructions for Use

  1. Slowly thaw the laminin stock solution at 4°C or on ice (Do not voltex).
  2. Dilute to an appropriate concentration using D-PBS (calcium- and magnesium-free) or medium.
  3. Coat cell culture vessel with the appropriate volume of diluted laminin solution.
  4. Incubate for 1 hour at 37℃ or overnight at 4℃.
  5. Carefully remove the laminin solution by aspiration.
  6. Wash the culture vessel 2-3 times with D-PBS (calcium- and magnesium-free) or a similar buffer.
  7. Plate cells.

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