StemSure® Gelatin Solution
StemSure® Gelatin Solution is a coating agent that can be used for cell culture dishes and multi-well plates.
It is composed of gelatin derived from porcine skin, and is prepared with D-PBS (-).
- Each lot is tested for its quality using murine ES cell line D3.
- It is prepared at the concentration of use, and is ready-to-use as a coating agent for culture vessels.
- Colony formation assay (using murine ES cell line D3)
- Alkaline phosphatase assay (using murine ES cell line D3)
- Sterility test
- Endotoxin test
- Mycoplasma test
Culturing Human iPS Cell Line 201B7
《Colony formation and ALP staining》
Multi-well plates were coated with StemSure® Gelatin Solution, and feeder sells were seeded onto the coated wells. Human iPS cell line 201B7 was cultured on the feeder cells, and the morphology of the iPS cells and positivity for ALP staining were examined.
D-MEM/Ham's F-12 + 20% StemSure® Serum Replacement + 2 mmol/L L-Glutamine + 1 x MEM Non-essential Amino Acids + 0.1 mmol/L StemSure® 2-Mercaptoethanol +1 x Penicillin-Streptomycin + 5 ng/mL bFGF
Culturing Murine ES Cell Line D3
Cloning efficiency for murine ES cell line D3 was compared for cells cultured on multi-well plates coated with StemSure® Gelatin Solution and those cultured on plates coated with an equivalent material from company A.
《Identifying expression of markers for undifferentiated cells》
Murine ES cell line D3 was cultured on a multi-well plate coated with StemSure® Gelatin Solution. After 16 passages, cells stained positive for markers of undifferentiated cells (Oct3/4, Sox2, Nanog).
《Identifying teratoma formation》
Murine ES cell line D3 was cultured for multiple passages on multi-well plates coated with StemSure® Gelatin Solution. Cells were then injected subcutaneously in immunocompromised mice. Teratomas formed subcutaneously, and various tissue types including nerve tissue (derived from ectoderm), cartilage tissue (derived from mesoderm), and luminal structures with ciliated epithelium (derived from endoderm) were found in the teratomas.
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