Collagen and Gelatin reagents

Cellmatrix^® Series (Collagen for Cell Culture)

FUJIFILM Wako offers five different types of Cellmatrix® collagen products, ensuring that you can find the ideal reagent for your specific research needs.

●Type Ⅰ-A ・Ideal for collagen-gel cultures. ・Perfect for microscopy due to its outstanding transparency.	●Type Ⅰ-P ・Recommended for both collagen-gel cultures and collagen-coating of culture surfaces. ・Forms softer gels than Type I-A. ・Pepsin-solubilized collagen from porcine tendons.
●Type Ⅰ-C ・Recommended for collagen-coating of culture vessels surfaces. ・Pepsin-solubilized collagen from porcine skin.	●Type Ⅲ ・Recommended for collagen-coating of culture vessels surfaces. ・Does not form gels. ・Type-III collagen from porcine skin.
●Type Ⅳ ・Recommended for collagen-coating of culture surfaces. ・Does not form gels. ・Type-IV collagen; purified from bovine anterior lens capsules using pepsin treatment.

Selection Guide for the Cellmatrix^® Series of Products (Reproduced from the website of Nitta Gelatin Inc.)

When you grow your cells…

Collagen-Gel Cell Culturing Kit / Concentrated Culture Media / Reconstitution Buffer

The Collagen-Gel Cell Culturing Kit is the right choice for researchers interested in implementing collagen cultures (2D/3D) using Cellmatrix^® I-A. This convenient starter kit contains all the reagents necessary for preparing collagen gel matrices.

• Collagen-Gel Cell Culturing Kit

Kit Contents

• Cellmatrix^® Type I-A　20mL　1bottle
• Concentrated Culture Media: Ham's F-12 and MEM　5mL each　1bottle each
• Reconstitution Buffer

Concentrated Culture Media

Specifically prepared for collagen-gel cultures
Available Media Types
o 10x Concentrates: Ham's F-12 medium, MEM with Hanks' balanced salt solution, DF medium (DME:F-12 = 1:1), and Medium 199.
o 5x Concentrates: DME medium and RPMI-1640.

Reconstitution Buffer

Specifically formulated for collagen-gel cultures.

Composition

• Sodium hydroxide (50mM)
• Sodium bicarbonate (260mM)
• HEPES (200mM)

beMatrix^® Series(Gelatin and Collagen for Tissue Engineering)

For researchers and industry professionals seeking higher levels of safety and quality in their materials for biomedical engineering and clinical applications.
Virus-inactivated collagen and gelatin with exceptionally low levels of endotoxins.

beMatrix^® Gelatin

Endotoxin Level: ≤10 EU/g
Nitta Gelatin Inc. has submitted a Device Master File (DMF) with the United States FDA for beMatrix^® Gelatin products.

Gelatin LS-H

• Alkaline-treated gelatin from porcine skin
• Gel Strength: High

Gelatin LS-W

• Alkaline-treated gelatin from porcine skin
• Gel Strength: Low

beMatrix^® Collagen

Collagen AT (Liquid)

• Endotoxin Level: ≤0.5 EU/mL
• Concentration: 3 mg/mL
• pH 3
• Acid-extracted collagen from porcine tendons

Collagen TE

• Endotoxin Level: ≤0.5 EU/mL
• Concentration: 5 mg/mL
• pH 3
• Pepsin-solubilized collagen from porcine tendons

Collagen FD (Lyophilized (Sponge). Size: Approx. 140 X 100 X 10 mm)

• Endotoxin Level: ≤100 EU/g
• Freeze-dried atelocollagen from porcine skin

MedGel^® Ⅱ Series (Gelatin-Based Matrix Materials for Controlled Release Formulations: An Advanced Version of the MedGel^® Series)

MedGel® Ⅱ is an improvement on the original MedGel^® ("TaBaTa Gel for controlled release" outside of Japan),
a bioabsorbable gelatin hydrogel for the sustained release of physiologically active substances.

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• [Advantages]
  ・Ready-to-use: Just add, dropwise, a solution of bioactive agents to be released at a controlled rate.
  ・Stabilizes encapsulated bioactive therapeutics.
  ・Enables local administration of drugs.
  ・Gelatin hydrogel prepared withoutchemical crosslinking agents.
  ・Sterilized by gamma irradiation.
  
  [Applications]
  ・Drug delivery research and the development of new drug delivery systems (DDSs).
  ・Basic and clinical research in cell therapy and regenerative medicine.
  
  [Improvements on the Original MedGel^®]
  ・Changed the method of sterilization to γ irradiation.
  ・Implemented a new crosslinking method that eliminates the need for chemical crosslinkers.

MedGel^® ("TaBaTa Gel for controlled release" outside of Japan) is a bioabsorbable gelatin based hydrogel that can release physiologically active substances in a gradual and continuous manner. It was developed based on research by Prof. Yasuhiko Tabata of the Institute for Frontier Medical Sciences at Kyoto University. MedGel^® is a water-insoluble polymer network formedby crosslinking gelatin. This polymeric network captures therapeutic agents primarily via electrostatic interactions. Following in vivo implantation MedGel^® - therapeutic reagent complex, MedGel^® is degraded by endogenous enzymes, allowing entrapped drugs to diffuse out of the network. MedGel^® is also designed to be completely degraded and absorbed in the body. This eliminates the need for implant removal surgery after the drugs are exhausted.

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• [Sheet Type]
  Nitta Gelatin Inc. offers two products (PI5 and PI9) with different isoelectric points. This enables you to select the hydrogel suitable for the isoelectric point (PI) property of your drug compound.

  [Particle Type]
  This form of hydrogel can be delivered via injection with a needle and syringe.

• Gelatin, GLS250 (Japanese Pharmacopoeia Grade)

  

  Features

  • Acid-treated gelatin from porcine skin
  • Powder

  Applications

  • Culture dish coating and other applications

• Gelatin, Reagent Grade

  Gelatin from porcine skin (type A) or bovine bones (type B) for high-strength gels.
  
  Applications

  • Basic laboratory protocols involving gelatin
  • Scaffold formation for tissue engineering
  • Coating of culture dishes and other cell culture substrates

GLS250 Gelatin Solution

Features

• Prepared from Japanese Pharmacopoeia-grade gelatin.
• Dissolved in water (Japanese Pharmacopoeia-grade distilled water for injection (WFI)).
• Sterilized by autoclaving.
• Concentration: 0.1%

Applications

• Cell culture
• Culture dish coating and other applications

Collagen BM

Features

• Pepsin-solubilized collagen from porcine skin
• Concentration: 5 mg/mL
• pH 3