H-ORAC Activity Assay Kit

Antioxidants such as polyphenols and carotenoids are substances that may protect cells against the effects of free radicals. There is an increasing interest to antioxidants in recent years due to a range of unique anti-aging properties. The Oxygen Radical Absorbance Capacity (ORAC) Assay is a reliable tool to evaluate the antioxidant capacity from foods extract. We provide a comprehensive analysis kit according to the standard of the National Agriculture and Food Research Organization, Japan.


Watanabe, J. et al. : Anal. Sci., 28, 159-165 (2012).


  • Possible to evaluate/test as same as standard procedures.
  • Easy to prepare reagents including buffers.

Measurement Principle


Analytical Method

Sample preparation


Analytical results

The same samples were analyzed by two labs in two ways (our assay kit and standard instrumental analysis), and the almost same results were obtained.


Watanabe, J. et al. : J. Food Sci., 79: C1665-C1671 (2014).

Kit Component

Item Pkg. Size Number
fluorescein 1 mL 1
Trolox 1 mL 2
APPH 15 mL 2
ferulic acid 100 mg 2
Assay Buffer 500 mL 1
(96 well transparent microplate)
- 2
plate seal - 4

Required lab equipment other than our kit

  • methanol (reagent grade)
  • acetic acid (reagent grade)
  • microplate reader
  • micropipette
  • conical tube
  • incubator (capable of keeping at 37°C)

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H-ORAC Activity Assay Kit

Extra microplate and plate seal

Author: S. Shibusawa

For research use or further manufacturing use only. Not for use in diagnostic procedures.

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