りん酸化タンパク質の高感度検出に

Phos-tag^® Mass Analytical Kit

Phos-tag^® Mass Analytical Kitは、MALDI-TOF/MSのサンプルと混合して使用します。ポジティブモードでりん酸化分子－ Phos-tag^® の複合体を検出し、通常では低いりん酸化分子の検出感度が向上します。

使用例：Phos-tag® - りん酸化LPA複合体の検出

プラス荷電の増大によりりん酸化LPAの検出感度が向上した。

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"Production of 1,2-Didocosahexaenoyl Phosphatidylcholine by Bonito Muscle Lysophosphatidylcholine/Transacylase", K. Hirano, H. Matsui, T. Tanaka, F. Matsuura, K. Satouchi, and T. Koike, J. Biochem., 2004, 136, 477-483.
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"A clean-up technology for the simultaneous determination of lysophosphatidic acid and sphingosine-1-phosphate by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry using a phosphate-capture molecule, Phos-tag", J. Morishige, M. Urikura, H. Takagi, K. Hirano, T. Koike, T. Tanaka, and K. Satouchi, Rapid Communications in Mass Spectrometry, 2010, 24, 1075-1084.