6 × His tag
FUJIFILM Wako supplies antibodies against 6xHis (histidine)-tag can be used to detect 6xHis-tagged fusion proteins. The specificity of the recognition site and application differ depending on the clone.
| Clone No. | Recognition site | Western blot | Immunoprecipitation | ELISA | Labelling | Product Number | ||
|---|---|---|---|---|---|---|---|---|
| N-terminal | C-terminal | N-terminal | C-terminal | |||||
| 9F2 | C-terminal | ND | +++++ | ND | ++ | - | - | 010-21861 |
| 9C11 | N/C-terminals | +++++ | ++++ | +++++ | ++ | ○ | - | 011-23091 |
| 21-48 | N/C-terminals | +++ | ++++ | +++ | +++ | ○ | - | 017-23211 |
| 28-75 | N/C-terminals | ++ | ++ | +++++ | +++++ | ○ | - | 014-23221 |
| 9F2 | C-terminal | ND | +++++ | - | - | - | HRP | 013-23171 |
| 9C11 | N/C-terminals | +++++ | ++++ | - | - | ◎ | HRP | 010-23181 |
Performance Comparison
Performance comparison of Western blotting
6xHis-Protein Z: expressed in HEK293T (1 x 105 cells)
Protein P-6xHis: expressed in CHO cells (1 x 105 cells)
Primary Antibody concentration: 1μg/mL
Secondary antibody: anti-mouse IgG antibody, 5,000-fold diluted solution
Detection sensitivity equal or superior to that of Competitive product B was confirmed.
Selection Guide
-
Which clone works best in the detection of N-terminal?
WB：9C11 、IP： 9C11, 28-75
Which clone works best in the detection of C-terminal?
WB：9F2 、IP：28-75
Which clone can recognize the both of N- and C-terminals and is best suited for Western blotting (WB)?
9C11
Which clone can recognize the both of N- and C-terminals and is best suited for immunoprecipitation (IP)?
28-75
-
Which clone is versatile and can handle both WB and IP?
21-48
Which clone works best with WB?
9C11
Which clone works best with IP?
28-75
Which is the cheapest clone to recognize the C-terminal alone ?
9F2
Product List
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Anti 6×His tag, Monoclonal Antibodies
Labeled Anti 6×His tag, Monoclonal Antibodies
Ni-NTA Agarose (Resin)
Ni-NTA Agarose (Cartridge)
Ni-NTA Agarose (Resin, Highly Cross-linked Agarose Beads)
6×His Peptides
Imidazole (Elution)
For research use or further manufacturing use only. Not for use in diagnostic procedures.
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