Anti CX3CR1 Antibodies

CX3CR1 is a receptor for CX3CL1, also known as fractalkine, and belongs to the GPCR family. In peripheral tissues, CX3CR1 is primarily expressed on the surface of macrophages, lymphocytes, and dendritic cells, and it participates in processes such as cell survival, proliferation, adhesion, and migration.

In the CNS, CX3CR1 is predominantly expressed in microglia and macrophages, serving as a marker for these cells. It plays a role in the development and maintenance of homeostasis within the CNS¹⁾. Notably, CX3CR1 knockout mice exhibit deficits in synaptic pruning by microglia, leading to abnormalities in synaptic transmission between neurons. This suggests that CX3CR1 is critical for synaptic pruning²⁾.

Anti CX3CR1, Guinea Pig is a polyclonal antibody against CX3CR1, derived from guinea pigs^3-4). It is suitable for use in multiplex immunohistochemical staining.

Antibody Information

Application Data

Immunohistochemistry

Species

Mouse

Site

Hippocampus

Sample

Frozen section

Primary antibody

Anti Iba1, Rabbit (for Immunocytochemistry)
(Product Number 019-19741) 1:500
Anti CX3CR1, Guinea Pig (this product) 1:800

NeuroTrace™435/455 Blue Fluorescent Nissl Stain (ThermoFisher Scientific, Inc.) was used for staining neurocytes.

[Data by courtesy of]
Dr. Miyata, Department of Applied Biology, Kyoto Institute of Technology

1. Wolf, Y. et al.: Front. Cell. Neurosci., 7, 26(2013).
   Microglia, seen from the CX3CR1 angle
2. Zhan, Y. et al.: Nat. Neurosci., 17(3), 400(2014).
   Deficient neuron-microglia signaling results in impaired functional brain connectivity and social behavior
3. Takagi, S. et al.: J. Neuroimmunology, 331, 74(2019).
   Microglia are continuously activated in the circumventricular organs of mouse brain
4. Kawai, S. et al.: Cell Biochem. Funct., 38(4), 392(2020).
   Transient increase of microglial C1q expression in the circumventricular organs of adult mouse during LPS‐induced inflammation