Albumin Quantitative Kits

Fujifilm Wako offers ELISA kits for various animal species and Turbidimetric Immuno Assay (TIA) reagents for automated measurement.

Albumin (Mouse,Rat) ELISA Kit

LBIS Mouse/Rat Albumin ELISA Kit is a sandwich ELISA system for quantitative measurement of mouse/Rat albumin. This is intended for research use only.

Features

  • Measurable in a short period (Total reaction time: 2 hours and 20 minutes)
  • A minute quantity of sample is measurable (5 μL with a standard method of operating)
  • Eco-friendly preservatives are used
  • All reagents are solution types and are ready to use
  • High measurement accuracy and reproducibility

Sample

Serum, Plasma or urine

  • Heparin is recommended to be used for collection of plasma.
  • Samples are diluted within the range of measurement with the accompanying buffering solution.
  • Rough indications of dilution are 10,000 to 50,000 times for a serum or plasma sample and 50 to 100 times for a urine sample.

Accuracy test

Precision of assay : Mean CV<10%
Reproducibility : Mean CV<10%

Specificity

The antibodies used in this kit are specific to albumin. Cross-reactivity of the kit is shown below.

  • Albumin Mouse ELISA Kit

    ※Cross reaction at 10000 ng/mL (Except FCS)

    Sample Cross reaction
    Rat Albumin Less than 5%
    Human Albumin N.D.
    Porcine Albumin N.D.
    Bovine Albumin N.D.
    10 % FCS N.D.
  • Albumin Rat ELISA Kit

    ※Cross reaction at 10000 ng/mL (Except FCS)

    Sample Cross Reaction
    Mouse Albumin Less than 5%
    Human Albumin N.D.
    Porcine Albumin N.D.
    Bovine Albumin N.D.
    10 % FCS N.D.

Standard curve

  • Mouse Albumin assay standard curve

    00840_img01.png

  • Rat Albumin assay standard curve

    00840_img02.png

Kit Components

1) Anti-albumin coated plate(8 x 12) 1 plate
2) Standard albumin mouse or rat(10 μg/mL) 1 vial
3) Buffer solution 60 mL
4) HRP-labeled anti-albumin antibody 100 μL
5) TMB Solution 12 mL
6) Stop Solution 12 mL
7) Wash stock Solution (x 10) 100 mL
8) Plate Seal 3 seals

References

Albumin Mouse ELISA Kit

  1. Mizukami, K. et al.: Naunyn. Schmiedebergs. Arch. Pharmacol., 391, 1319(2018)
  2. Rhee, H. et al.: Kidney. Res. Clin. Pract., 37, 222(2018)
  3. Ishikawa, H. et al.: Biosci. Biotechnol. Biochem., 82, 515(2018)

Albumin Rat ELISA Kit

  1. Kanno, M. et al.: Nephrology., 23, 1046(2018)
  2. Ishibashi, Y. et al.: Horm. Metab., 48, 613(2016)
  3. Takeshige, Y. et al.: Hypertens., 39, 415(2016)

Turbidimetric Immuno Assay Kit (TIA Kit)

Features

  • Measurement range Mouse/rat 6.17 to 500 μg/mL
  • Samples are urine or serum
  • Special antibodies for each species (monkey/mouse/rat albumin) are used for a specific reaction
  • Measurable in a short period (10 minutes) with a generalpurpose biochemical autoanalyzer
  • Wide measurement range with superior reproducibility
  • It cannot be affected by manipulation because it is measured with an autoanalyzer
  • Parameters for each autoanalyzer made by domestic manufacturers are being prepared (except a part of them)

Accuracy test

Mouse, rat in common

Precision of assay : Mean CV<7.5%
Reproducibility : Mean CV<7.5%

Kit Components

1) Reagent 1 (Buffer) 18.5 mL
2) Reagent 2 (Anti-mouse or rat albumin solution) 6.6 mL
3) Standard mouse or rat albumin* 2.0 mL
4) Diluent solution for standard albumin 4.0 mL

*Concentration of standard mouse or rat albumin is indicated on its bottle label.

Assay procedure

For Hitachi 7180 autoanalyzer
*Note that operation of autoanalyzer is different depending on the type of machines

00840_img03.png

References

LBIS Mouse Urinary Albumin Assay Kit

  1. Haku, S. et al.: Biomed. Res. Int., 2018, 2817045(2018)
  2. Iwashita, Y. et al.: Am. J. Physiol. Renal. Physiol., 310, F1206(2016)
  3. Zhu, K. et al.: Free. Radic. Biol. Med., 83, 21(2015)

LBIS Rat Urinary Albumin Assay Kit

  1. Tatsuyori, M. et al.: Eur. J. Pharmacol., 756, 85(2015)
  2. Ruixia, M. et al.: Int. J. Clin. Exp. Pathol., 8, 14063(2015)
  3. Sakai, M. et al.: Eur. J. Pharmacol., 761, 109(2015)

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Albumin (Mouse,Rat) ELISA Kit

Albumin (Bovine) ELISA Kit

TIA reagent for automatic measuring instrument

For research use or further manufacturing use only. Not for use in diagnostic procedures.

Product content may differ from the actual image due to minor specification changes etc.

If the revision of product standards and packaging standards has been made, there is a case where the actual product specifications and images are different.

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