Orexin A ELISA Kit Wako

Given that the level of orexin A in the cerebrospinal fluid is significantly decreased in patients with narcolepsy, a type of sleep disorder, orexin A is thought to play an important role in controlling sleep functions. The level of orexin A has been measured conventionally using radioimmunoassay. However, due to the need for radioisotopes (RI), the measurements could only be performed at dedicated institutions with proper equipment.

This product is an ELISA kit that enables simple measurements of orexin A from human cerebrospinal fluid, as well as rat cerebrospinal fluid, serum, and plasma without the need for radioisotope.

• No need for RIs
• High sensitivity (4.69-300 pg/mL)
• Only a small amount of samples is needed (25 μL)

• Performance

  Standard curve range	4.69～300 pg/mL
  Samples	human cerebrospinal fluid, rat cerebrospinal fluid, rat plasma, rat serum※１
  Necessary amount of sample	25 μL
  Duration of measurement	About 20 hours
  Within-run reproducibility	CV＜5 ％
  Between-run reproducibility	CV＜16 ％

  ※１: The levels of orexin A in normal human plasma and serum, as well as in wild-type mouse plasma and serum, are undetectable using the kit. The level may be high enough to be detected if orexin A was added or Orexin A was overexpressed.

• Standard curve

  

Spike-and-recovery