Microglia Remover

Microglia Remover is anionic liposomes suspension containing clodronic acid. When this product is phagocytosed by microglia as foreign substances, clodronic acid is released within the cells, causing cell death. By encapsulating clodronic acid in liposomes, it is taken up into microglia more efficiently than clodronic acid alone. Microglia remover (for Control) (sold separately, Product Number 131-19511) is also available as a control for experiments.

Features

• Microglia can be removed in approximately 1-3 days after administration
• Less expensive than commercial microglia removal feeds

Product Information

Protocol

Intracerebral infusion

1. 1-2 μL Microglia Remover or Microglia Remover (for Control) are infused into the cerebral parenchyma.
2. Assess the depletion of microglia in 1-3 days post-infusion.

Application Data

Microglia removal in mouse cerebral cortex

After inserting a 33 G needle into the mouse cerebral cortex, 1 µL of Microglia Remover (for Control) or Microglia Remover (this product) was injected, respectively, and sampled after 3 days.

Animal

Mouse (C57BL6/J)

Site

Cerebral cortex

Sample

Frozen section

Antibody Concentration

Primary Antibody

Anti Iba1, Rabbit (for immunocytochemistry) (Product Number 019-19741) 1:500

Secondary Antibody

Alexa Fluor^® 488 AffiniPure Goat Anti-Rabbit IgG (H+L) (Jackson ImmunoResearch Product Number 111-545-144) 1:500

Data by courtesy of
Dr. Miyata, Department of Applied Biology, Kyoto Institute of Technology

[Result]

In the control group, microglia (or macrophages) accumulated in large numbers at the site of injury.
On the other hand, the “Microglia Remover” group showed inhibition of the accumulation of microglia in the injured area and observed a decrease or regression in the number of microglia in the surrounding area.