Exosome Standard: For the positive control in Exosome research

Exosomes, from COLO201 cells, purified

  • banner_exosome_consulting_EN.png
  • banner_exosome_guidebook_EN.png

This product is highly purified exosomes isolated from a cell culture supernatant of COLO201 cells through PS affinity method, using Tim4 protein which specifically binds phosphatidylserine (PS) displayed on the surface of exosomes.

Features

  • High purity
  • Long-term stability
  • Ready-to-use liquid format
  • CD9, CD63 and CD81 positive

Specifications

  • Physical State:Liquid
  • Constituent:Exosomes, TBS, EDTA, Polymer as a stabilizer
  • Concentration:10 μg/mL*
    *The value is corrected protein concentration by a CD63 signal.

Detecting exosome surface markers by Western blot

10 ng of “Exosomes, from COLO201 cells, purified” was loaded on each well and SDS-PAGE was performed under non-reducing conditions. Then, canonical exosome surface markers such as CD9, CD63 and CD81 were detected using anti-CD9, anti-CD63 and anti-CD81 antibodies.

01689_img01.png
Primary antibodies
  • Anti CD9, Monoclonal Antibody (1K) (Product code: 014-27763)
  • Anti CD63, Monoclonal Antibody (3-13) (Product code: 012-27063)
  • Anti CD81, Monoclonal Antibody (17B1) (Product code: 011-27773)
Secondary antibody
  • HRP conjugated anti-Mouse IgG

CD9, CD63 and CD81 are highly expressed on “Exosomes, from COLO201 cells, purified” .

Next, 10 ng of “Exosomes, from COLO201 cells, purified” and five on-market Exosomes were loaded on each well and SDS-PAGE was performed under non-reducing conditions. Then, CD63 was detected using anti-CD63 antibodies.

01689_img02.png
Lane No.

① “Exosomes, from COLO201 cells, purified”
② Competitor 1: Exosomes, from A549 cells
③ Competitor 2: Exosomes, from HEK293 cells
④ Competitor 3: Exosomes, from A549 cells
⑤ Competitor 4: Exosomes, from COLO1 cells
⑥ Competitor 5: Exosomes, from HCT116 cells

Primary antibody

Anti CD63, Monoclonal Antibody (3-13) (Product code: 012-27063)

Secondary antibody

HRP conjugated anti-Mouse IgG

Our “Exosomes, from COLO201 cells, purified” only showed a CD63 signal compared to five on-market Exosomes in detecting the presence of CD63.

Particle size analysis using NanoSight

Comparison of particle size analysis between “Exosomes, from COLO201 cells, purified” and Exosomes from Competitor 1 using NanoSight.

  • 01689_img03.png
  • 01689_img04.png

“Exosomes, from COLO201 cells, purified” have expected particle size distribution.

Effect of repeated freeze-thaw on exosome structure

Structure of “Exosomes, from COLO201 cells, purified” which have been frozen at -20℃ and thawed at room temperature 15 times was observed by an electron microscope.

01689_img05.png

“Exosomes, from COLO201 cells, purified” was maintained their structure even they have undergone 15 times freeze-thaw cycles.

Product List

  • Open All
  • Close All

Related Product List

  • Open All
  • Close All

Exosome extraction / purification reagent

Exosome Flowcytometry kit

Exosome Blocking reagent

Exosome Marker antibody

For research use or further manufacturing use only. Not for use in diagnostic procedures.

Product content may differ from the actual image due to minor specification changes etc.

If the revision of product standards and packaging standards has been made, there is a case where the actual product specifications and images are different.

The prices are list prices in Japan.Please contact your local distributor for your retail price in your region.

Please contact us via the inquiry form.