CultureSure™ Small Molecules

What is A419259?

A419259 is an inhibitor of Src family kinases. Src is involved in the production of tyrosine kinases that specifically phosphorylate tyrosine residues of proteins. Src family kinases are considered to play important roles in cell development, differentiation and proliferation signaling pathways.

※ Tested for endotoxin, tested negative for mycoplasma

Product Description

• Assay (HPLC): min. 98.0%
• Appearance: white to pale yellowish red, crystalline powder to powder
• Solubility: soluble in DMSO
• Tested negative for mycoplasma
• Endotoxin: less than 0.1 EU/mg
• Storage: room temperature (under inert gas)

What is A-83-01?

A-83-01 is a selective inhibitor of ALK4, ALK5, and ALK7. It blocks phosphorylation of Smad2/3 and inhibits TGF-β-induced epithelial-to-mesenchymal transition. This product has little to no effect on bone morphogenetic protein type I receptors, p38 MAP kinase, and extracellular signal-regulated kinases. A-83-01 is known to help rat iPS cellscan be long-term cultured in a state maintaining the undifferentiated state.

※ Tested for endotoxin, tested negative for mycoplasma

Product Description

• Assay (HPLC): min. 98.0%
• Appearance: white to yellow, crystalline powder to powder or mass
• Solubility: soluble in DMSO
• Tested negative for mycoplasma
• Endotoxin: less than 0.01 EU/mg
• Storage: -20°C in the dark (under inert gas)

References

Li, W., et al. : Cell Stem Cell, 4, 16 (2009).
Katsuda ,T., et al. : Cell Stem Cell, 20, 41 (2017).
Okae, H., et al. : Cell Stem Cell, 22, 50 (2018).

What is ALK5 Inhibitor Ⅱ?

ALK5 Inhibitor Ⅱ is a TGF-βR I kinase/ALK5 inhibitor. It can be used as a substitute for Sox2 or c-Myc when generating mouse iPS cells.

※ Tested for endotoxin, tested negative for mycoplasma

Product Description

• Assay (HPLC): min. 98.0%
• Appearance: white to yellowish brown, crystals to powder
• Solubility: soluble in DMSO
• Tested negative for mycoplasma
• Endotoxin: less than 0.05 EU/mg
• Storage: 2-10℃ in the dark (under inert gas)

References

Maherali, N., et al. : Curr. Biol., 19, 1718 (2009).

What is CHIR99021?

CHIR99021 is a highly selective inhibitor of GSK-3β. The product does not exhibit cross-reactivity against CDKs.
CHIR99021 has been used to maintain effectively undifferentiated ES cells in combination with PD0325901.
It has also been reported to induce differentiation into various cell types, including cardiomyocytes, neurons, and multiple renal progenitor cells. Furthermore, cells induced from human iPS cells using SB431542, CHIR99021, and Dorsomorphin have been shown to exhibit significantly enhanced efficiency and speed in differentiation toward the target cell types.

※ Tested for endotoxin, tested negative for mycoplasma

Product Description (Powder Form)

• Assay (HPLC): min. 97.0%
• Appearance: white to pale brown, crystalline powder to powder
• Solubility: soluble in DMSO and methanol
• Tested negative for mycoplasma
• Endotoxin: less than 0.25 EU/mg
• Storage: -20°C in the dark

Product Description (Liquid Form)

• Appearance: liquid
• Concentration: dissolved in DMSO, 10 mmol/L
• Sterilized with 0.1 μm filter
• Tested for sterility
• Tested negative for mycoplasma
• Endotoxin: less than 2 EU/mL
• Storage: -20°C in the dark
• No animal-derived components were used in the raw materials and in the manufacturing processes

Reference

Ying, QL., et al.: Nature, 453, 519 (2008).
Fujimori, K., et al.: Stem Cell Reports, 9, 1 (2017).
Laco, F., et al.: Stem Cell Reports, 10, 1851 (2018).
Lian, X., et al.: Proc. Natl. Acad. Sci., 109, E1848 (2012).
Tsujimoto, H., et al.: Cell Rep., 31 (2020).
Fujimori, K., et al.: Stem Cell Reports, 9, 1 (2017).

What is CKI-7 Dihydrochloride?

CKI-7 dihydrochloride is one of the isoquinoline sulfonamide, and is an ATP-competitive inhibitor that selectively inhibit casein kinase 1 (CK1). As a serine/ threonine kinase, CK1 regulates various processes such as nuclear export, cell cycle, cell division, and apoptosis. CKI-7 dihydrochloride is known for induce retinal progenitor cells from human ES and iPS cells in combination with SB431542 and Y-27632 under serum-free and feeder-free conditions.

※ Tested for endotoxin, tested negative for mycoplasma

Product Description (Liquid Form)

• Appearance: liquid
• Concentration: dissolved in water, 3 mmol/L
• Sterilized with 0.1 μm filter
• Tested for sterility
• Tested negative for mycoplasma
• Tested for endotoxin: less than 1 EU/mL (for the first lot)
• Storage: -20°C in the dark
• No animal-derived components were used in the raw materials and in the manufacturing processes

Reference

Takahashi, M. et al.: J. Cell Sci., 122, 3169 (2009).

What is DAPT?

DAPT is a γ-secretase inhibitor that inhibits Notch signaling. It induces neural differentiation from ES cell embryoid bodies and preferentially induces cone photoreceptor subtypes in retinal cell differentiation. It is also used as a component of medium when differentiation of brain organoid.

※ Tested for endotoxin, tested negative for mycoplasma

Product Description

• Assay (HPLC): min. 99.0%
• Appearance: white to slightly yellowish brown, crystalline powder to powder or mass
• Solubility: soluble in DMSO
• Tested negative for mycoplasma
• Endotoxin: less than 0.25 EU/mg
• Storage: -20℃

References

Crawford, TQ., et al. : Dev. Dyn., 236, 886 (2007).
Nelson, BR., et al. : Dev. Biol., 304, 479 (2007).
Zerti, D., et al. : Stem Cells, 38, 45 (2020).

What is Dorsomorphin?

This is a pyrazolopyrimidine compound with cellular permeability. It acts as a potent, selective, reversible, and ATP-competitive inhibitor of AMPK.
It induces neural differentiation from human iPS cells, and cardiomyocytes from mouse ES cells.
It has been reported that human brainstem organoids can be derived from human iPS/ES cells when used in combination this with several other small molecule compounds.

※ Tested for endotoxin, tested negative for mycoplasma

Product Description

• Assay (HPLC): min. 98.0%
• Appearance: white to yellow, crystals to powder
• Solubility: soluble in DMSO
• Tested negative for mycoplasma
• Endotoxin: less than 0.25 EU/mg
• Storage: -20℃ (under inert gas)

References

Chen, Y., et al. : Methods Mol. Biol., 1919, 59 (2019).
Hao, J., et al. : PLOS ONE, 3, e2904 (2008).
Nobuyuki, E., et al. : Front Neurosci., 14, 538 (2020).

What is Forskolin?

Forskolin is an activator of adenylate cyclase. It induces differentiation of mesenchymal stem cells into neurons when used in conjunction with bFGF. It is also used in culture liver organoids.

※ Tested for endotoxin, tested negative for mycoplasma

Product Description

• Assay (HPLC): min. 98.0%
• Appearance: white to slightly yellowish brown, crystalline powder to powder or mass
• Solubility: soluble in DMSO
• Tested negative for mycoplasma
• Endotoxin: less than 0.25 EU/mg
• Storage: 2-10℃

References

Jang, S., et al. : BMC Cell Biol., 11, 25 (2010).

What is IWP-2?

IWP-2 inactivates porcupine (Porcn), a membrane-bound o-acyltransferase (MBOAT), and suppresses palmitoylation of Wnt proteins. As a result, it blocks phosphorylation of Wnt-dependent Lrp-6 receptor and Dvl2, and accumulation ofβ-catenin. It is known that it promotes differentiation of human iPS cells into cardiomyocytes.

※ Tested negative for mycoplasma

Product Description

• Assay (HPLC): min. 98.0%
• Appearance: white to pale brown, crystals to powder
• Solubility: soluble in DMSO
• Tested negative for mycoplasma
• Storage: 2-10 °C in the dark (under inert gas)

Reference

Minami, I. et al.: Cell Rep., 2, 1448 (2012).

What is IWR-1-endo?

IWR-1-endo is an inhibitor of Wnt signaling. IWR-1 stabilizes the complex composed of Axin2, Apc, Ck1, and Gsk3β that degrades β-catenin. As a result, it promotes degradation of β-catenin.

※ Tested for endotoxin, tested negative for mycoplasma

Product Description

• Assay (HPLC): min. 98.0%
• Appearance: white to pale yellow, crystalline powder to powder
• Solubility: soluble in DMSO
• Tested negative for mycoplasma
• Endotoxin: less than 0.25 EU/mg
• Storage: -20 °C in the dark (under inert gas)

What is KY03-I?

KY03-I is an inhibitor of Wnt. It is known that KY02111 induces differentiation of human ES and iPS cells into cardiomyocytes. Compared with KY02111, KY03-I induces differentiation of ES/ iPS cells into cardiomyocytes in a more efficient manner and at a lower concentration.

※ Tested for endotoxin, tested negative for mycoplasma

Product Description

• Assay (HPLC): min. 98.0%
• Appearance: white to pale gray, crystalline powder to powder
• Solubility: soluble in DMSO
• Tested negative for mycoplasma
• Endotoxin: less than 0.01 EU/mg
• Storage: -20 °C in the dark (under inert gas)

Reference

Minami, I. et al.: Cell Rep., 2, 1448 (2012).

What is PD0325901?

PD0325901 is a MAPK inhibitor with potent antitumor activity.
It induces phosphorylation of MAPK, inhibition of its activation and inhibition of tumor cell proliferation. PD0325901 inhibits MAPK activity without adverse reactions.
Addition of this product and CHIR99021 to a culture medium results in efficient culture of mouse ES cells.
It has been reported that use of this product with SB431542 and thiazovivin not only improves reprogramming efficiency 200 folds or more but also accelerates reprogramming speed.

※ Tested for endotoxin, tested negative for mycoplasma

Product Description

• Assay (HPLC): min. 99.0%
• Appearance: white to pale brown, crystals to powder
• Solubility: soluble in DMSO
• Tested negative for mycoplasma
• Endotoxin: less than 0.25 EU/mg
• Storage: -20℃ (under inert gas)

References

Lin, T., et al. : Nat. Methods, 6, 805 (2009).
Ying, QL., et al. : Nature, 453, 519 (2008).
Koehler, KR., et al. : Nat. Protoc., 9, 1229 (2014).

What is Purmorphamine?

Purmorphamine is an agonist of hedgehog signaling. Induces differentiation of mesenchymal progenitor cells and preosteoblast cells into osteoblasts.

※ Tested for endotoxin, tested negative for mycoplasma

Product Description

• Assay (HPLC): min. 98.0%
• Appearance: white to pale brown or bluish purple, crystalline powder to powder or mass
• Solubility: soluble in DMSO
• Tested negative for mycoplasma
• Endotoxin: less than 0.25 EU/mg
• Storage: -20℃

References

Wu, X., et al. : Chem. Biol., 11, 1229 (2004).

What is SAG?

SAG is a cell-permeable Smoothened (Smo) agonist that activates the Sonic Hedgehog (Shh) signaling pathway. It has been reported to induce differentiation of ES/iPS cells into neurons, chondrocytes, and three-dimensional spinal cord tissue.

Product Description

• Assay (HPLC): min. 99.0%
• Appearance: white to pale brown, crystals to powder
• Solubility: soluble in DMSO
• Tested negative for mycoplasma
• Endotoxin: less than 0.25 EU/mg
• Storage: -20℃ (under inert gas)

Reference

Lau, ST., et al. : Gastroenterology, 157 (6), 1556 (2019).
Kanke, K., et al. : Stem Cell Reports, 2 (6), 751 (2014).
Xue, W., et al. : iScience, 26 (1), 104 (2023).

What is SANT-1?

SANT-1 is a Sonic hedgehog signal inhibitor. It is known that it used in the process of inducing differentiation into pancreatic islet beta cells.

Product Description

• Appearance:：white to yellow, crystals to powder
• Assay (HPLC): min. 99.0%
• Solubility: soluble in DMSO
• Endotoxin: less than 0.25 EU/mg
• Tested negative for mycoplasma

Reference

Leonardo, VC., et al.: Stem Cell Reports., 12(2), 351 (2019).
Federica, F., et al.: Front. Cell Dev. Biol., 10, 967765 (2022).　

What is SB431542?

SB431542 is a selective inhibitor of TGF-β superfamily type 1 activin receptor-like kinase (ALK) and inhibits ALK4, 5, and 7. It is known that it stimulates proliferation, differentiation and sheet formation of ES cells-derived endothelial cells. It is also known that the use of SB431542 with thiazovivin and PD0325901 improves reprogramming efficiency to more than 200 times and accelerates reprogramming speed.

※ Tested for endotoxin, tested negative for mycoplasma

Product Description (Powder Form)

• Assay (HPLC): min. 98.0%
• Appearance: white to slightly yellowish brown, crystalline powder to powder
• Solubility: soluble in DMSO
• Tested negative for mycoplasma
• Endotoxin: less than 0.05 EU/mg
• Storage: -20 °C in the dark

Product Description (Liquid Form)

• Appearance: liquid
• Concentration: dissolved in DMSO, 5 mmol/L
• Sterilized with 0.1 μm filter
• Tested for sterility
• Tested negative for mycoplasma
• Tested for endotoxin: less than 2 EU/mL (for the first lot)
• Storage: -20°C in the dark
• No animal-derived components were used in the raw materials and in the manufacturing processes

References

Ogawa, K., et al. : J. Cell. Sci.,120, 55 (2007).
Lin T., et al. : Nat. Methods, 6, 805 (2009).
Fujimori, K., et al. : Stem Cell Reports., 9, 1 (2017).
Okae, H., et al. : Cell Stem Cell, 22, 50 (2018).

What is 3,3',5-Triiodo-L-thyronine Sodium Salt (T3)?

It is one of the thyroid hormones. It signals by binding to nuclear receptors and regulating the expression of a wide range of gene products.
It promotes differentiation of oligodendrocyte progenitor cells or maturation of human iPS cell-derived cardiomyocytes. Thyroid hormone signaling has also been reported to act on cone subtype determination in retinal cell differentiation.
※ Tested for endotoxin, tested negative for mycoplasma

Product Description

• Assay (HPLC): min. 98.0%
• Appearance: white to pale yellowish brown or reddish brown, crystalline powder to powder
• Solubility: soluble in DMSO
• Tested negative for mycoplasma
• Endotoxin: less than 0.25 EU/mg
• Storage: -20℃ (under inert gas)

References

Marta, CB., et al. : J. Neurosci. Res., 53, 251 (1998).
Yang, X., et al. : J. Mol. Cell. Cardiol., 72, 296 (2014).
Eldred, KC., et al. : Science, 362, eaau6348 (2018).

What is TTNPB?

TTNPB is a selective retinoic acid receptor agonist inhibitor. It is known that it used in the process of inducing differentiation into renal cells, nephron progenitor cells, and pancreatic islet beta cells.

Product Description

• Appearance:White to nearly white, crystals to powder
• Assay (HPLC): min. 99.0%
• Solubility: soluble in DMSO
• Endotoxin: less than 0.25 EU/mg
• Tested negative for mycoplasma
• Storage: -20°C (under inert gas)

References

Toyohara, T., et al.: Stem Cells Transl. Med., 4(9), 980 (2015).
Toyoda, T., et al.: Stem Cell Reports, 9(2), 419 (2017).

What is Y-27632?

Y-27632 is a selective and potent inhibitor of ROCK. It induces various activities such as contraction of vascular smooth muscle cells via ROCK signaling pathways. Furthermore, it suppresses the cell death of of ES and iPS cells at cell dispersion, and that it improves cell survival after cryopreservation. [ROCK：Rho-associated coiled-coil forming kinase / Rho-linked kinase/ serine/threonine protein kinase]

※ Tested for endotoxin, tested negative for mycoplasma

Product Description (Powder Form)

• Assay (HPLC): min. 98.0%
• Appearance: white to pale yellow, crystalline powder to powder
• Solubility: soluble in water and ethanol
• Specific rotation [α]_D²⁰ (c=0.5, CH₃OH): +2 to +10°
• Tested negative for mycoplasma
• Endotoxin: less than 0.25 EU/mg
• Storage: -20 °C in the dark (under inert gas)

Product Description (Liquid Form)

• Appearance: liquid
• Concentration: dissolved in water, 10 mmol/L
• Sterilized with 0.1 μm filter
• Tested for sterility
• Tested negative for mycoplasma
• Endotoxin: less than 3EU/mL
• Storage: -20°C in the dark
• No animal-derived components were used in the raw materials and in the manufacturing processes

Our Y-27632 products lineup (including GMP products)

References

Uehata, M., et al. : Nature, 389, 990 (1997).
Sakamoto, K., et al. : J. Pharmacol. Sci., 92, 56 (2003).
Nishimaru, K., et al. : J. Pharmacol. Sci., 92, 424 (2003).
Watanabe, K., et al. : Nat. Biotechnol., 25, 681 (2007).
Martin-Ibanez, R., et al. : Hum. Reprod., 23, 2744 (2008).
Claassen, DA., et al. : Mol. Reprod. Dev., 76, 722 (2009).
Kawamata, M., et al. : Proc. Natl. Acad. Sci. USA., 107, 14223 (2010).
Ito, H., et al. : Liver Int., 32, 592 (2012).
Katsuda,T., et al. : Cell Stem Cell, 20, 41 (2017).
Okae, H., et al. : Cell Stem Cell, 22, 50 (2018).
Ogawa, K., et al. : Sci. Rep., 8, 3615 (2018).
Ayabe, H., et al. : Stem Cell Reports., 11, 306 (2018).